Trends in Phytochemical Research
type: channel
description: "Trends in Phytochemical Research (TPR)", is a prominent journal that publishes original research articles, review papers, short communications, letter to editor, technical notes, and book reviews. It covers a wide range of topics including the characterization and processing of essential oils, analytical assessments of bioactive compounds, natural products chemistry, phytochemical evaluations of essential oils and extracts, and their antioxidant, antibacterial, antimicrobial, antifungal properties. The journal also explores areas such as food chemistry and the biological activities of naturally occurring compounds. The abbreviated form of this journal is "Trends Phytochem. Res.". TPR is an open access and a free of charge journal for all authors all over the world. In other words, there is no article processing charge for each accepted and subsequently published article in the journal. At present, it publishes four issues per year. “Trends in Phytochemical Research (TPR)”, an international open access and quarterly journal that has been regularly published since March 2017. The average time between submission and final decision is six to eight weeks and the average time between acceptance and publication is about one month. The submitted papers are also tracked via reliable "Plagiarism Checker" like "iThenticate plagiarism checker" to make sure their originality. The other objective of our journal is to publish the highest quality articles and disseminate to all readers through our "Open Access" mode. The published articles are not recommended for commercial use. Journal Abbreviation: Trends Phytochem. Res. Journal Title Acronym: TPR Owner organisation: Islamic Azad University- Shahrood Branch Publisher: Islamic Azad University-Shahrood Branch, Co-publishing: OICC Press, UK Latest News: We would like to inform all authors and contributors that, effective 1st November 2025, an Article Processing Charge (APC) will apply to all new submissions, manuscripts submitted o…
publisher: OICC Press
language: en
publication name: Trends in Phytochemical Research
issn: 2588-3623
items: Synthesis and Characterization of a Rosemary Extract-Based Nano-Ointment for Enhanced Diabetic Wound Healing in Rats
items: Biochemical and Molecular Responses of Tomato (Lycopersicon esculentum Mill.) to Abiotic Stresses: Investigating the Interplay of Oxygen-Evolving Complex and Phytochemical Modulation
items: Role of Thymus vulgaris L. (Thyme) in Facilitating Postpartum Placental Expulsion: A Systematic Review of Studies Published Between 2015 and 2025
items: https://oiccpress.com/tpr/article/view/19777
items: https://oiccpress.com/tpr/article/view/18870
items: The first report on the screening of the GC/MS profiles of the hydrodistilled essential oils and volatile components from the aerial parts of Verbascum sinuatum L. (Scrophulariaceae)
items: Deep eutectic solvent-based ultrasonic assisted extraction of polysaccharides and antioxidants from Astragalus hamosus L. seedpod
items: Effects of NaCl stress on growth, photosynthesis, and phytochemical composition of creeping savory (Satureja spicigera (C. Koch) Boiss.)
items: In vitro protective effects of Helichrysum cymosum (L.) D.Don (Asteraceae) on TM4 Sertoli cell
items: Hypericum perforatum L. (Hypericaceae): Cultivation, diversity of biochemical composition, antimicrobial activities, active biomolecules and action mode-A review
items: Unveiling the potential antidiabetic, antidyslipidemic, and antimicrobial effects of methanolic root extracts of Nymphaea pubescens Willd. plant: Evidence from in-vitro and in-vivo approaches
items: Exploration of In-vitro Antioxidant and In-vivo Analgesic, Antipyretic, and Antidiarrheal Properties of the Ethanolic Extract of Cyclea barbata (Miers) (Menispermaceae)
items: Extraction and Quantification of Formononetin from RedClover (Trifolium pratense L.) and its Anticancer Effects on Hepatocellular Carcinoma Cells
items: Chemical compositions and yield of essential oil from sage (Salvia officinalis L.) under organic inputs,L-phenylalanine, and varying moisture levels
items: Naphthalene acetic acid as a potent elicitor of coordinated defense responses and terpenoid metabolism reprogramming in the medicinal succulent Euphorbia trigona Mill. (Euphorbiaceae)
items: Seasonal assessment of the best harvesting months of Bangladeshi Piper betle L. (Piperaceae) leaves: Insights into polyphenols and antioxidant activity using multivariate techniques
items: Characterization, quantification and antioxidant potential of phenolic compounds in Brachychiton rupestris (T.Mitch. ex Lindl.) K. Schum. leaves
items: Integrated analysis of anatomy, phytochemistry, and biological potentials of Marrubium vulgare L. extracts
items: Scavenging Free Radical Potential and Electron Donor Capacity of Piper betle L. (Piperaceae): Comparative Analysis of Antioxidant Activity Across Geographic Locations
items: Isolation and characterization of phytoconstituents and exploration of in vitro and in vivo biological activities of Anaxagorea luzonensis A. Gray (Annonaceae)
items: In vitro assessment of the antihyperglycemic property of Prosopis farcta J. F. Macbr and Rheum ribes L. and phytochemical profiling of the most active extract
items: Phenolic composition and antioxidant capacity of leaves infusions of Annona muricata L. (Annnonaceae) from two regions of Mexico
items: Green synthesis of iron nanoparticles via Rhizoclonum riparium (Roth) Harvey: A promising nanotherapy against the promastigote forms of Leishmania donovani parasites
items: Bibliometric analysis of Murraya koenigii (L.) Spreng research: Trends and hotspots
items: Genotype × environment interaction, rhizome yield stability and selection for region specific stable genotypes in turmeric (Curcuma longa L.)
items: Phytochemical investigation and isolation of new compounds from the stems of Tinospora cordifolia Miers
items: Influence of extraction methods on total phenolic, flavonoids and antioxidant activity of Thymus kotchyanus L. extract in Semnan Province, Iran
items: Extraction, purification, characterization and antioxidant activities of heat-resistance phenolic compounds from lemon pulp
items: Isolation and identification of growth promoting endophytes from Artemisia annua L. and its effects on artemisinin content
items: Quantification of β-carotene, aucubin content, their associations and contribution to other economic traits in Plantago germplasm

Synthesis and Characterization of a Rosemary Extract-Based Nano-Ointment for Enhanced Diabetic Wound Healing in Rats
type: item
description: Diabetes is characterized by chronic inflammation, impaired angiogenesis, and delayed tissue regeneration. In this study, a chitosan/rosemary extract/gallic acid nanostructure was synthesized, characterized, and formulated as a topical nano-ointment for diabetic wound healing. Characterization by TEM, FT-IR, UV–Vis, and XRD confirmed the successful formation of a nearly spherical nanostructure with an average size of approximately 40 nm and verified interactions among its components. Twenty-eight male Wistar rats were assigned to seven experimental groups, including healthy, wound control, vehicle treated group, and treatment groups receiving chitosan, rosemary extract, gallic acid, or the nano-ointment. After diabetes induction and wound creation, topical treatment was administered, and wound tissues were evaluated histopathologically using Hematoxylin–Eosin staining. Compared with the diabetic control, the nano-ointment significantly reduced inflammatory cell infiltration, enhanced collagen deposition, and promoted epithelial regeneration. These findings indicate that the chitosan/rosemary extract/gallic acid nano-ointment represents a promising strategy for accelerating diabetic wound healing.
subject: Original Articles
  topic: section
subject: Anti-inflammatory
  topic: keywords
subject: Characterization
  topic: keywords
subject: Chitosan
  topic: keywords
subject: Diabetic wound
  topic: keywords
subject: Gallic acid
  topic: keywords
subject: Nano-ointment
  topic: keywords
subject: Rosemary extract)
  topic: keywords
creator: Razieh Sanjari
creator: Mohsen Forozanfar
creator: Fatemeh Honarasa
creator: Mohammad Amin Edalatmanesh
creator: Heydar Aqababa
rights: Copyright (c) 2026 Razieh Sanjari, Mohsen Forozanfar, Fatemeh Honarasa, Mohammad Amin Edalatmanesh, Heydar Aqababa (Author) https://creativecommons.org/licenses/by/4.0
date: 2026-04-30
publication date: 2026-04-30
doi: 10.57647/tpr.2026.1001.02

Biochemical and Molecular Responses of Tomato (Lycopersicon esculentum Mill.) to Abiotic Stresses: Investigating the Interplay of Oxygen-Evolving Complex and Phytochemical Modulation
type: item
description: Abiotic stresses such as drought, salinity, heat, cold, and cadmium impair photosynthesis in tomato by disrupting the oxygen-evolving complex (OEC) of photosystem II (PSII). In this study, tomato plants (Lycopersicon esculentum Mill.) were exposed to these six stress treatments, and their responses were evaluated using chlorophyll <span style="color: #6c6c6c; font-family: Arial, sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; white-space: normal; background-color: #ffffff; text-decoration-thickness: initial; text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none;"> <strong class="rQesXe MPyX" style="font-family: 'Google Sans', Arial, sans-serif; font-size: 16px; font-weight: bold; margin: 0px; text-decoration: none; border-bottom: 0px rgb(10, 10, 10);" data-sfc-cp="" data-sfc-root="ep" data-copy-service-computed-style="font-family: "Google Sans", Arial, sans-serif; font-size: 16px; font-weight: 700; margin: 0px; text-decoration: none; border-bottom: 0px rgb(10, 10, 10);">\(a\) fluorescence measurement (JIP-test), expression of OEC-related genes (PsbO, PsbP, PsbQ, PsbR), phytochemical content and antioxidant metabolism. The results showed that all stress treatments reduced PSII efficiency, disrupted thylakoid organization, and downregulated OEC genes, notably under heat and cadmium, which reduced the photosynthetic performance index (PIabs) by 47.14% and 44.29%, respectively. In addition, stress exposure decreased chlorophyll content and altered the ascorbate-glutathione cycle, with the most pronounced reduction in ascorbate content occurring under cadmium stress, reaching 62.95% compared to the control. At the biochemical level, total phenolic compounds and flavonoids generally increased…
subject: Original Articles
  topic: section
subject: Abiotic stress
  topic: keywords
subject: Gene expression
  topic: keywords
subject: Oxygen-evolving complex (OEC)
  topic: keywords
subject: Phytochemicals
  topic: keywords
subject: Photosystem II (PSII)
  topic: keywords
subject: Tomato (Lycopersicon esculentum Mill.)
  topic: keywords
creator: Niloofar Amirinezhad
creator: Mojtaba Jafarinia
creator: Ali Akbar Ghotbi-Ravandi
rights: Copyright (c) 2026 Niloofar Amirinezhad, Mojtaba Jafarinia, Ali Akbar Ghotbi-Ravandi (Author) https://creativecommons.org/licenses/by/4.0
date: 2026-04-30
publication date: 2026-04-30
doi: 10.57647/tpr.2026.1001.01

Role of Thymus vulgaris L. (Thyme) in Facilitating Postpartum Placental Expulsion: A Systematic Review of Studies Published Between 2015 and 2025
type: item
description: Thymus vulgaris L. (thyme) is a medicinal herb traditionally used in Iranian and Mediterranean medicine for its antioxidant, anti-inflammatory, and antimicrobial properties. This systematic review, conducted in accordance with PRISMA guidelines, evaluated evidence regarding thyme’s role in postpartum placental expulsion. Studies published between 2015 and 2025 were searched; 180 records were identified, 58 full texts were assessed, and 35 studies met the inclusion criteria. Thymol, carvacrol, and rosmarinic acid promoted uterine contractions by modulating oxytocin receptors and calcium channels. In animal studies, thyme oil at 50 mg/kg accelerated placental expulsion and enhanced antioxidant capacity. Early human evidence indicated a shorter third stage of labor and reduced postpartum hemorrhage. Additional antioxidant, anti-inflammatory, and antimicrobial activities may support postpartum recovery. Despite promising preclinical and clinical findings, evidence remains limited by the lack of large randomized trials, standardized preparations, and safety data during lactation, warranting further investigation before routine obstetric use.
subject: Review Articles
  topic: section
subject: Thymus vulgaris L.
  topic: keywords
subject: Placental expulsion
  topic: keywords
subject: Uterotonic
  topic: keywords
subject: Thymol
  topic: keywords
subject: Ethnobotany
  topic: keywords
subject: Phytochemistry
  topic: keywords
creator: Najmeh Sheikhi
creator: Ramin Tavakoli
creator: Seyyed Ali Shariatzadeh
creator: Zahra Bazi
creator: Reyhaneh Nouraei
creator: Abdolvahab Moradi
rights: Copyright (c) 2026 Najmeh Sheikhi, Ramin Tavakoli, Seyyed Ali Shariatzadeh, Zahra Bazi, Reyhaneh Nouraei, Abdolvahab Moradi (Author) https://creativecommons.org/licenses/by/4.0
date: 2026-04-30
publication date: 2026-04-30
doi: 10.57647/tpr.2026.1001.05

https://oiccpress.com/tpr/article/view/19777
type: item
title: Chemical Composition and Chemotypic Variability of Tanacetum persicum (Boiss.) Mozaff. (Asteraceae) Essential Oils: Insights from Microwave-Assisted Hydrodistillation in Combination with GC-MS Analysis
description: This study investigates the essential oil (EO) compositions of Tanacetum persicum (Boiss.) Mozaff. aerial parts collected from five alpine regions in Kerman Province, Iran, namely Hazar, Lalehzar, Jupar, Plovar, and Bahr Aseman mountains. Utilizing microwave-assisted hydrodistillation (MAHD) combined with gas chromatography-mass spectrometry (GC-MS), a total of 36 volatile constituents were identified, representing between 99.3% and 99.9% of the total oil profiles. The characterized chemical profiles revealed a predominant accumulation of oxygenated monoterpenes (OMs) across all sites. Accordingly, major constituents exhibited significant quantitative variation depending on geographic origin, notably borneol (13.8%-20.1%), bornyl acetate (10.1%-19.1%), linalool (11.4%-19.9%), 1,8-cineole (8.5%-13.1%), and cumin aldehyde (5.3%-14.1%). These findings highlight substantial chemotypic diversity and intraspecific metabolic adaptation driven by geographical and environmental heterogeneity across the high-altitude habitats of Kerman Province.
subject: Original Articles
  topic: section
subject: Asteraceae
  topic: keywords
subject: Borneol
  topic: keywords
subject: Bornyl acetate
  topic: keywords
subject: Chemotypes
  topic: keywords
subject: Heatmap analysis
  topic: keywords
subject: Hierarchical cluster analysis (HCA)
  topic: keywords
subject: Oxygenated monoterpenes (OMs)
  topic: keywords
subject: Tanacetum persicum (Boiss.) Mozaff.
  topic: keywords
creator: Majid Mohammadhosseini
rights: Copyright (c) 2026 Majid Mohammadhosseini (Author) https://creativecommons.org/licenses/by/4.0
date: 2026-04-30
publication date: 2026-04-30
doi: 10.57647/tpr.2026.1001.06

https://oiccpress.com/tpr/article/view/18870
type: item
title: Chemical composition of the essential oils and volatile fractions from the aerial parts of Crocus sativus L.(Iridaceae) using microwave assisted hydrodistillation (MAHD) and headspace solid phase microextraction(HS-SPME) combined with gas chromatography-massspectrometric (GC-MS) analysis
description: In this report, chemical composition of the essential oils and volatile fractions from the aerial parts of Crocus sativus L. (Iridaceae) was investigated using microwave-assisted hydrodistillation (MAHD) and headspace solid-phase microextraction (HS-SPME), followed by gas chromatography-mass spectrometry (GC-MS) analysis on DB-5 and HP-5MS columns. Twenty constituents were identified by matching their mass spectra, retention indices, and co-injection with reference standards. In the MAHD-derived essential oil, seventeen compounds accounted for 97.3% of the total composition, with safranal (37.2%) as the major component, followed by 4-ketoisophorone (11.2%), hexadecane (9.1%), hexadecanoic acid (8.1%), tetradecanoic acid (7.5%), nonacosane (6.2%), and n-eicosane (5.1%). The HS-SPME volatile fraction exhibited a higher safranal content (63.5%), accompanied by hexadecanoic acid (10.2%), ethyl hexadecanoate (6.7%), tetradecanoic acid (5.7%), and isophorone (3.0%). These findings reveal pronounced method-dependent variations in the volatile profiles, underscoring the dominance of safranal and highlighting the potential of saffron aerial parts as an alternative source of valuable aroma compounds.
subject: Original Articles
  topic: section
subject: Crocus sativus L.
  topic: keywords
subject: Iridaceae; Microwave Assisted Hydrodistillation (MAHD)
  topic: keywords
subject: Headspace Solid Phase Microextraction (HS-SPME)
  topic: keywords
subject: Volatile fractions
  topic: keywords
subject: Safranal (2,6,6-Trimethyl-1,3-cyclohexadien-l-carboxaldehyde)
  topic: keywords
creator: Majid Mohammadhosseini
rights: Copyright (c) 2026 Majid Mohammadhosseini (Author) https://creativecommons.org/licenses/by/4.0
date: 2025-12-31
publication date: 2025-12-31
doi: 10.57647/tpr.2025.0904.19

The first report on the screening of the GC/MS profiles of the hydrodistilled essential oils and volatile components from the aerial parts of Verbascum sinuatum L. (Scrophulariaceae)
type: item
description: This study elucidates the chemical composition of the essential oil and volatile fractions from the aerial parts of Verbascum sinuatum L. using classical hydrodistillation (CHD) and headspace-solid phase microextraction (HS-SPME) techniques, identifying 23 compounds via GC-MS analysis. In the CHD essential oil, non-terpene hydrocarbons (NH) predominated at 64.8%, followed by oxygenated diterpenes (OD) at 14.2%, sesquiterpene hydrocarbons (SH) at 8.0%, and oxygenated sesquiterpenes (OS) at 7.8%. The principal constituents were hexadecanoic acid (28.9%), phytol (14.2%), and linoleic acid (7.3%), accounting for 94.8% of the total. On the other hand, HS-SPME profiles emphasized NH (70.1%) and OS (19.1%), with hexadecanoic acid (49.3%), (E)-phytol acetate (8.1%), n-heneicosane (4.1%), and spathulenol (4.0%) comprising 92.5% overall. These findings reveal method-dependent variations in terpenoid and fatty acid content, highlighting CHD's efficacy in extracting higher quantities of oxygenated diterpenes and HS-SPME's sensitivity to volatiles, offering insights into phytochemical diversity for potential pharmacological applications.
subject: Original Articles
  topic: section
subject: Classical hydrodistillation (CHD), Essential oils, GC/MS, Headspace-solid phase microextraction (HS-SPME), Scrophulariaceae, Verbascum sinuatum L.
  topic: keywords
creator: Majid Mohammadhosseini
rights: Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0
date: 2025-09-30
publication date: 2025-09-30
starting page: 212
ending page: 222
doi: 10.57647/tpr.2025.0903.18

Deep eutectic solvent-based ultrasonic assisted extraction of polysaccharides and antioxidants from Astragalus hamosus L. seedpod
type: item
description: In this study, the efficiency of various solvents and techniques was compared for the extraction of polysaccharides and antioxidants from Astragalus hamosus. Among twenty solvents and four extraction techniques, ultrasonic-assisted extraction using choline chloride-urea (a deep eutectic solvent, DES) demonstrated the best performance. Extraction variables were optimized by the response surface methodology (RSM), and Box-Behnken design (BBD). Extraction yield and total carbohydrate content (TCC) were 44.5% and 2.90 mgGlu/gdw, respectively. Rhamnose, mannose and galactose (molar ratio: 0.34:1.18:1.0) were major monosaccharides in the extracted polysaccharides. Six antioxidant assays—total phenolic content (TPC), total flavonoid content (TFC), DPPH assay, ferric-reducing antioxidant power (FRAP), trolox equivalent antioxidant capacity (TEAC), and reducing power assay (RPA)—were used to evaluate antioxidant capacity. Additionally, different extraction methods, including ultrasonic-assisted, microwave-assisted, maceration, and Soxhlet extractions were compared.
subject: Original Articles
  topic: section
subject: Astragalus hamosus L., Antioxidants, Deep eutectic solvent, Leguminosae, Polysaccharides, Ultrasonic assisted extraction, Response surface methodology
  topic: keywords
creator: Reza Tabaraki
creator: Fariba Fatahi
rights: Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0
date: 2025-09-30
publication date: 2025-09-30
starting page: 201
ending page: 211
doi: 10.57647/tpr.2025.0903.17

Effects of NaCl stress on growth, photosynthesis, and phytochemical composition of creeping savory (Satureja spicigera (C. Koch) Boiss.)
type: item
description: The study examined the effects of NaCl treatments (50, 100, and 150 mM) on various morpho-physiological, photosynthetic and phytochemical traits of creeping savory, a wild medicinal plant, in a greenhouse experiment based on a completely randomized design (CRD) with three replications. Increasing NaCl concentration led to a linear decline in growth parameters, with the highest reductions at 150 mM. Leaf, shoot, and root fresh and dry weights decreased significantly under all NaCl levels. Photosynthetic index improved at 50 mM but declined at 150 mM NaCl. Chlorophyll index also decreased significantly at 150 mM. Essential oil percentage increased under 50 and 100 mM NaCl, however dropped at 150 mM. While α-pinene, β-bisabolene, terpinene-4-ol, methyl ether thymol, methyl ether carvacrol, trans-caryophyllene, p-cymene, and γ-terpinene were decreased in plants treated with NaCl, the percentages of thymol and carvacrol were increased.
subject: Original Articles
  topic: section
subject: Essential oil; Medicinal plants; Photosynthetic indices; Phytochemicals; Salt stress; Satureja spicigera (C. Koch) Boiss.
  topic: keywords
creator: Borzou Yousefi
creator: Hoshang Rahmati
creator: Hooshmand Safari
creator: Jalal Ghaderi
rights: Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0
date: 2025-09-30
publication date: 2025-09-30
starting page: 191
ending page: 200
doi: 10.57647/tpr.2025.0903.16

In vitro protective effects of Helichrysum cymosum (L.) D.Don (Asteraceae) on TM4 Sertoli cell
type: item
description: Helichrysum cymosum (L.) D.Don phytocompounds play an integral role in treating various diseases due to their bioactive properties. This study evaluated the protective effects of two H. cymosum extracts (aqueous and 70% ethanol) on cell viability, morphological changes, antioxidant enzyme activities (SOD, GSH and TAC) and ROS production on TM4 Sertoli cells. Results showed no significant effect on cell viability after 24 h but significantly decreased after 48 h (p < 0.05). Cell structure integrity was maintained at all concentrations compared to the positive control (10% DMSO). Antioxidant enzyme activities SOD, GSH and TAC had good scavenging properties, although no statistically difference was recorded. ROS production was maintained with both extracts, albeit with no significant differences. H. cymosum extracts may contain potent bioactive components such as flavonoids that work synergistically to mitigate OS-induced infertility in males. Further investigations including in vivo experiments, are recommended to validate their use in herbal supplements and drug discovery.
subject: Original Articles
  topic: section
subject: Antioxidant capacity, Asteraceae, Cytotoxicity, Glutathione, Helichrysum cymosum (L.) D.Don, Phytocompounds, Superoxide dismutase
  topic: keywords
creator: Achasih Quinta Nkemzi
creator: Chinyerum Sylvia Opuwari
creator: Okobi Eko Ekpo
creator: Oluwafemi Omoniyi Oguntibeju
rights: Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0
date: 2025-09-16
publication date: 2025-09-16
starting page: 169
ending page: 180
doi: 10.57647/tpr.2025.0903.14

Hypericum perforatum L. (Hypericaceae): Cultivation, diversity of biochemical composition, antimicrobial activities, active biomolecules and action mode-A review
type: item
description: This study analyses the knowledge on the cultivation, diversity, and variation of phytochemical constituents of essential oils and crude extracts from Hypericum perforatum. Equally, we presented a detailed discussion of the antimicrobial activities against pathogens and viruses, and the implicated biomolecules. A complete and organized literature search was carried out in the basic databases. According to the reviewed papers, Hypericum perforatum contains considerable amounts of chemical constituents, including phenolic, flavonoid, and terpene compounds, which are diverse and variable depending on the geography of plants, parts used, and extraction methods. These chemical constituents demonstrated serious inhibitory effects against fungi, bacteria, and viruses.
subject: Review Articles
  topic: section
subject: Antimicrobial activities; Bioactive compounds; Essential oils; Hypericum perforatum L.; Hypericaceae; Mechanism; Phytochemical constituents
  topic: keywords
creator: Hajar Afqir
creator: Saadia Belmalha
creator: Jihane Laarifi
creator: Ayoub Farihi
creator: Soumaya Baataoui
creator: Brahim Bourkhiss
creator: Hassane Tahiri
creator: Mohammed Ouhssine
rights: Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0
date: 2025-09-15
publication date: 2025-09-15
starting page: 154
ending page: 168
doi: 10.57647/tpr.2025.0903.13

Unveiling the potential antidiabetic, antidyslipidemic, and antimicrobial effects of methanolic root extracts of Nymphaea pubescens Willd. plant: Evidence from in-vitro and in-vivo approaches
type: item
description: This study investigated the therapeutic potential of methanolic root extracts of Nymphaea pubescens Willd. with a focus on their antidiabetic, antilipidemic, and antimicrobial properties. The methanolic root extracts of the N. pubescens at higher doses (500 mg/kg) exhibited a pronounced serum blood glucose-lowering activity (p < 0.001) and also demonstrated remarkable outcomes in body weight loss (p < 0.01), closely resembling the effects of the standard metformin. The same experiment displayed a noticeable reduction in low-density lipoprotein cholesterol (LDL-C) and total cholesterol (TC) levels (p < 0.001), along with a notable elevation in high-density lipoprotein cholesterol (HDL-C) levels (p < 0.01). Furthermore, the plant extract at 500 mg/kg unveiled significant antimicrobial activities (p < 0.001) against both Staphylococcus aureus and Aspergillus niger and also exhibited significant (p < 0.01) inhibition of Escherichia coli. In summary, these findings highlight the multifaceted therapeutic potential of N. pubescens root extracts, warranting further investigation for their possible development into novel therapeutic agents.
subject: Original Articles
  topic: section
subject: Antidiabetic; Antihyperlipidemic; Antimicrobial; In vitro; In vivo; Nymphaea pubescens Willd.; Nymphaeaceae
  topic: keywords
creator: Sahid Ahmad Santo
creator: Tanmay Deb
creator: Md. Abdus Salam
creator: Arafat Miah
creator: Md. Saddam Hussain
creator: Anamika Datta
creator: Mohammad Nurul Amin
creator: Mohammad Sarowar Uddin
creator: Mohammad Safiqul Islam
creator: Md. Shalahuddin Millat
rights: Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0
date: 2025-09-30
publication date: 2025-09-30
starting page: 181
ending page: 190
doi: 10.57647/tpr.2025.0903.15

Exploration of In-vitro Antioxidant and In-vivo Analgesic, Antipyretic, and Antidiarrheal Properties of the Ethanolic Extract of Cyclea barbata (Miers) (Menispermaceae)
type: item
description: The ethanolic leaf extract of Cyclea barbata (Miers) (Thangbandri), a medicinal plant widely used in traditional medicine, was investigated for its phytochemical composition and pharmacological activities. The extract contained significant levels of polyphenols, including total phenolics (31.60 mg GAE/g), tannins (69.31 mg GAE/g), and flavonoids (50.41 mg QE/g). Its antioxidant potential was demonstrated through DPPH, hydroxyl radical, and nitric oxide scavenging assays, with IC₅₀ values of 35.44, 43.73, and 51.42 μg/mL, respectively. In vivo studies showed significant dose-dependent analgesic activity in the acetic acid-induced writhing model, achieving 57.26% and 88.71% inhibition at 250 and 500 mg/kg, respectively (p < 0.001). The extract also exhibited notable antipyretic and antidiarrheal effects, reducing fever and suppressing defecation by up to 80.74%. These findings indicate that C. barbata leaves are a promising source of bioactive phytochemicals with antioxidant, analgesic, antipyretic, and antidiarrheal properties.
subject: Original Articles
  topic: section
subject: Analgesic
  topic: keywords
subject: Antidiarrheal
  topic: keywords
subject: Antioxidant
  topic: keywords
subject: Antipyretic
  topic: keywords
subject: Cyclea barbata
  topic: keywords
subject: Menispermaceae
  topic: keywords
subject: Polyphenols
  topic: keywords
creator: Md. Rasul Karim
creator: Al Mamun Shohag
creator: Md. Sabbir Hossain
creator: Md. Shamim
creator: Md Anisur Rahman
creator: Tonima Sultan Toma
creator: Md. Saiful Islam
rights: Copyright (c) 2026 Md. Rasul Karim, Al Mamun Shohag, Md. Sabbir Hossain, Md. Shamim, Md Anisur Rahman, Tonima Sultan Toma, Md. Saiful Islam (Author) https://creativecommons.org/licenses/by/4.0
date: 2026-04-30
publication date: 2026-04-30
doi: 10.57647/tpr.2026.1001.04

Extraction and Quantification of Formononetin from RedClover (Trifolium pratense L.) and its Anticancer Effects on Hepatocellular Carcinoma Cells
type: item
description: Formononetin, a major isoflavone derived from Trifolium pratense L., exhibits promising anticancer effects in various tumor models; however, its activity against hepatocellular carcinoma (HCC) remains underexplored. In the current report, formononetin was extracted and purified from the aerial parts of T. pratense L., and UPLC-ESI-MS/MS analysis confirmed a concentration of 800 ± 15 ppm in the standardized extract. Treatment of HepG2 cells with formononetin (1-640 µM) for 24 to 72 hours resulted in a dose- and time-dependent reduction in cell viability, as measured by the MTT assay. Specifically, 20 µM reduced cell survival to approximately 10%, while 40 µM induced near-complete cytotoxicity at 72 h. Exposure to 10 µM formononetin over five days suppressed viability, as assessed by trypan blue exclusion, indicating primarily cytostatic effects. Clonogenic assays demonstrated a significant decrease in colony formation, from approximately 75 to 55 colonies (p < 0.01). DAPI staining revealed chromatin condensation, nuclear shrinkage, and apoptotic bodies consistent with intrinsic apoptosis after 48 h. These findings demonstrate potent anticancer activity through proliferative arrest and induction of apoptosis, warranting further in vivo studies in HCC.
subject: Original Articles
  topic: section
creator: Hamed Abdolkarimi
creator: Hossein Sadeghi
creator: Mojtaba Jafarinia
creator: mohsen Forouzanfar
rights: Copyright (c) 2026 Hamed Abdolkarimi, Hossein Sadeghi, Mojtaba Jafarinia, mohsen Forouzanfar (Author) https://creativecommons.org/licenses/by/4.0
date: 2025-12-31
publication date: 2025-12-31
doi: 10.57647/tpr.2025.0904.20

Chemical compositions and yield of essential oil from sage (Salvia officinalis L.) under organic inputs,L-phenylalanine, and varying moisture levels
type: item
description: The effects of the foliar application of L-phenylalanine, organic inputs, and different soil moisture conditions on the phytochemistry of the essential oil of sage (Salvia officinalis L.) were investigated. This field study was done according to a split-plot in time and place design in West of Iran in two experimental years. According to essential oils analysis by GC-FID and GC/MS, the major compounds of the sage essential oil were oxygenated monoterpenes, including camphor (32-39%), 1,8-cineole (25-31%), α-thujone (19-23%), and β-thujone (3-4%). The highest values of these oxygenated monoterpenes were obtained from the optimum soil moisture and foliar spraying with 50% L-phenylalanine under an organic farming system, which significantly influenced the quantity and quality of the biologically active compounds of sage. In conclusion, the foliar spray of L-phenylalanine under organic inputs, and their interaction could ameliorate the dry matter yield and essential oil compounds of sage in environmentally friendly agriculture conditions.
subject: Original Articles
  topic: section
subject: Amino acid
  topic: keywords
subject: Camphor
  topic: keywords
subject: 1,8-Cineole
  topic: keywords
subject: Essential oil
  topic: keywords
subject: Environmentally friendly
  topic: keywords
subject: GC/MS; Sage
  topic: keywords
subject: α-Thujone
  topic: keywords
creator: Safoura Hasanabadi
creator: M.Reza Ardakani
creator: Abdolah Ghasemi Pirbalouti
creator: Farzad Paknejad
creator: Davoud Habibi
rights: Copyright (c) 2026 Safoura Hasanabadi, M.Reza Ardakani, Abdolah Ghasemi Pirbalouti, Farzad Paknejad, Davoud Habibi (Author) https://creativecommons.org/licenses/by/4.0
date: 2025-12-31
publication date: 2025-12-31
doi: 10.57647/tpr.2025.0904.24

Naphthalene acetic acid as a potent elicitor of coordinated defense responses and terpenoid metabolism reprogramming in the medicinal succulent Euphorbia trigona Mill. (Euphorbiaceae)
type: item
description: Plant growth regulators (PGRs) are effective tools for enhancing phytochemical production in medicinal plants. This study investigated the elicitation efficacy of gibberellin (GA3) and auxins (IAA, IBA, NAA) at 250 mg/L on the medicinal succulent Euphorbia trigona Mill. (Euphorbiaceae). After seven months, NAA treatment significantly increased root biomass and uniquely triggered a coordinated defense response. This was evidenced by the significant upregulation of phenylalanine ammonia-lyase (PAL) and increased activities of the antioxidant enzymes peroxidase and superoxide dismutase. Consequently, NAA induced a substantial accumulation of defense-related metabolites, most strikingly a five-fold increase in terpenoids, along with elevated flavonoids and flavonols. GC-MS profiling suggested an NAA-induced reprogramming of the terpenoid profile. The results demonstrate that unlike GA3 or other auxins, NAA is the most effective elicitor for enhancing bioactive compounds in E. trigona through a coordinated defense network.
subject: Original Articles
  topic: section
subject: Coordinated response
  topic: keywords
subject: Elicitation
  topic: keywords
subject: Euphorbiaceae
  topic: keywords
subject: Euphorbia trigona Mill
  topic: keywords
subject: Metabolic reprogramming
  topic: keywords
subject: Naphthalene acetic acid (NAA)
  topic: keywords
subject: Phenylalanine ammonia-lyase
  topic: keywords
subject: Secondary metabolism
  topic: keywords
subject: Terpenoids
  topic: keywords
creator: Hakimeh Rezaei
creator: Aryan Sateei
creator: Tahereh A. Aghajanzadeh
creator: Mehdi Ebadi
rights: Copyright (c) 2026 Hakimeh Rezaei, Aryan Sateei, Tahereh A. Aghajanzadeh, Mehdi Ebadi (Author) https://creativecommons.org/licenses/by/4.0
date: 2025-12-31
publication date: 2025-12-31
doi: 10.57647/tpr.2025.0904.21

Seasonal assessment of the best harvesting months of Bangladeshi Piper betle L. (Piperaceae) leaves: Insights into polyphenols and antioxidant activity using multivariate techniques
type: item
description: This study evaluated monthly and maturity stage variations in polyphenols [total phenol (TP) and total flavonoid (TF)] and antioxidant activity (AA) in Piper betle L. leaves collected between 2021 and 2024. TP and TF were quantified using the Folin-Ciocalteu and aluminum chloride methods, respectively, while AA was measured by DPPH and ABTS assays. TP and TF ranged from 23.56 ± 3.51 to 58.81 ± 26.15 mg GAE/g and 8.93 ± 0.29 to 35.55 ± 0.14 mg QE/g, respectively. IC50 values for DPPH and ABTS varied from 0.45 ± 0.010 to 1.70 ± 0.000 and 0.24 ± 0.001 to 0.87 ± 0.001 (mg AAE/mL), subsequently. The highest TP, TF, and AA were found in March, corresponding to low rainfall (18 mm), moderate temperature (25.35 °C), and humidity (70.5%). Principal component analysis (PCA) and hierarchical cluster analysis (HCA) identified three seasonal clusters. March is recommended as the optimal harvest month to maximize bioactive potential for food, pharmaceutical, and cosmetic applications.
subject: Original Articles
  topic: section
subject: Piper betle L.
  topic: keywords
subject: Monthly variation
  topic: keywords
subject: Bangladesh
  topic: keywords
subject: Polyphenols
  topic: keywords
subject: Antioxidant activity
  topic: keywords
creator: Naim Islam
creator: Jahangir Alam
creator: Manobendra Nath Mohanta
creator: Mst. Khadiza Khatun
creator: Hillol Roy
creator: Fairuz Islam
creator: Sunjida Akhter
creator: Mst. Fakid Sharmin Sristy
creator: Md. Mosharef Hossain Bhuiyan
creator: Kyong Su Kim
creator: Md. Atikul Islam
rights: Copyright (c) 2026 Naim Islam, Jahangir Alam, Manobendra Nath Mohanta, Mst. Khadiza Khatun, Hillol Roy, Fairuz Islam, Sunjida Akhter, Mst. Fakid Sharmin Sristy, M. Mosharef Hossain Bhuiyan, Kyong Su Kim, M. Atikul Islam (Author) https://creativecommons.org/licenses/by/4.0
date: 2025-12-31
publication date: 2025-12-31
doi: 10.57647/tpr.2025.0904.22

Characterization, quantification and antioxidant potential of phenolic compounds in Brachychiton rupestris (T.Mitch. ex Lindl.) K. Schum. leaves
type: item
description: The studies conducted to investigate the pharmacological potential or the chemical composition of Brachy chiton rupestris leaves were very limited. The present study aims to isolate, identify and quantify the major phenolics in B. rupestris leaves and to evaluate their antioxidant potential. Methanol (85.0%) extract of B. rupestris leaves was prepared and successively partitioned with petroleum ether, dichloromethane, ethylacetate and n-butanol, respectively. The bioactive compounds in the ethyl acetate and n-butanol fractions were separated chromatographically, and their structures were characterized. Their concentrations were determined using HPLC-DAD, and their antioxidant potential was evaluated against DPPH. Caffeic acid, p-coumaric acid, nicotiflorin, methyl gallate, isoquercetin, rutin, quercetin and kaempferol were the major phytochemicals. Methyl gallate exhibited the most potent antioxidant activity with an IC50 of 18.19 ± 0.161 µg/mL. B. rupestris could be considered a promising source of antioxidants which suggests its application in nutraceuticals and therapeutic formulations.
subject: Original Articles
  topic: section
subject: Antioxidant
  topic: keywords
subject: Brachychiton rupestris (T.Mitch. ex Lindl.) K. Schum.
  topic: keywords
subject: Caffeic acid
  topic: keywords
subject: HPLC-DAD
  topic: keywords
subject: Malvaceae
  topic: keywords
subject: Methyl gallate
  topic: keywords
subject: Nicotiflorin
  topic: keywords
creator: Heba Raafat Mohamed
creator: Eman Ahmed El-Wakil
creator: Maher Mahmoud El-Hashash
creator: El-Sayed Saleh Abdel-Hameed
rights: Copyright (c) 2025 Heba Raafat Mohamed, Eman Ahmed El-Wakil, Maher Mahmoud El-Hashash, El-Sayed Saleh Abdel-Hameed (Author) https://creativecommons.org/licenses/by/4.0
date: 2025-09-27
publication date: 2025-09-27
doi: 10.57647/tpr.2025.0902.10

Integrated analysis of anatomy, phytochemistry, and biological potentials of Marrubium vulgare L. extracts
type: item
description: This study reports on the phytochemical and bioactive characteristics of Marrubium vulgare L., harvested in northeast Algeria. After extraction using hydrodistillation and solvents with increasing polarity, 0.05% essential oil and 5.9% apolar crude extract were obtained. The plant presented a significant amount of phytochemicals like polyphenols (126.17 mg GAE/g), flavonoids (42.08 mg QE/g), tannins (45.07 mg TAE/g) which explained the high antioxidant power, evidenced by in vitro assays (FRAP, H2O2, OH• and cyclic voltammetry). Furthermore, the antimicrobial test against various microorganisms (Staphylococcus aureus, Salmonella sp, Shigella sp. Aspergillus niger, Aspergillus flavus) showed that the methanolic extract was broadly effective against all tested strains. However, among the less polar extracts, activity varied: essential oils showed moderate activity, dichloromethanic extract was active against most bacteria and petroleum ether extract showed significant antibacterial effects. These compelling results underscore the unique and significant therapeutic potential of the different extracts derived from Marrubium vulgare L.
subject: Original Articles
  topic: section
subject: Antimicrobial activity
  topic: keywords
subject: Antioxidant activity
  topic: keywords
subject: Bioactive compounds
  topic: keywords
subject: Marrubium vulgare L.
  topic: keywords
subject: Phytochemistry
  topic: keywords
subject: Trichomes
  topic: keywords
creator: Souad Djellali
creator: Rachid Sahraoui
creator: Yasmine Abdelouahed
creator: Mohamed amine Benyahia
creator: Mira Chribet
rights: Copyright (c) 2026 Souad Djellali, Rachid Sahraoui, Yasmine Abdelouahed, Mohamed amine Benyahia, Mira Chribet (Author) https://creativecommons.org/licenses/by/4.0
date: 2025-12-31
publication date: 2025-12-31
doi: 10.57647/tpr.2025.0904.23

Scavenging Free Radical Potential and Electron Donor Capacity of Piper betle L. (Piperaceae): Comparative Analysis of Antioxidant Activity Across Geographic Locations
type: item
description: Reactive oxygen species (ROS) induce oxidative stress, leading to disease and cellular injury. Betel, or Piper betle L., is rich in phenolic and flavonoid compounds which has antioxidant properties. This study evaluated the free radical scavenging potential and electron-donation capacity of P. betle L. leaf extracts from various locations to determine their antioxidant efficacy. The ethyl acetate extract of betel leaves from Cianjur demonstrated the highest electron-donating ability-measured using the ferric reducing antioxidant power (FRAP) method, and the greatest free radical scavenging activity-assessed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, with values of 611.603 ± 26.565 and 2.231 ± 0.072 mg AEAC/g, respectively. Betel leaves from different regions demonstrated stronger potential as electron donors to enhance antioxidant capacity than as free radical scavengers. Overall, P. betle leaves are recognized as a potent natural source of antioxidants.
subject: Original Articles
  topic: section
subject: Antioxidant assays
  topic: keywords
subject: DPPH
  topic: keywords
subject: FRAP
  topic: keywords
subject: Piper betle L.
  topic: keywords
subject: Piperaceae
  topic: keywords
subject: Reactive oxygen species (ROS)
  topic: keywords
subject: Total flavonoid content (TFC)
  topic: keywords
subject: Total phenolic content (TPC)
  topic: keywords
creator: Rika Hartati
creator: Jeanne Belle Leo Fiona
creator: Atina Rizkiya Choirunnisa
creator: Hegar Pramastya
creator: Defri Rizaldy
creator: Irda Fidrianny
rights: Copyright (c) 2026 Rika Hartati, Jeanne Belle Leo Fiona, Atina Rizkiya Choirunnisa, Hegar Pramastya, Defri Rizaldy, Prof. Dr. Irda Fidrianny (Author) https://creativecommons.org/licenses/by/4.0
date: 2026-04-30
publication date: 2026-04-30
doi: 10.57647/tpr.2026.1001.03

Isolation and characterization of phytoconstituents and exploration of in vitro and in vivo biological activities of Anaxagorea luzonensis A. Gray (Annonaceae)
type: item
description: This study aimed to evaluate the α-glucosidase inhibition potential of Prosopis farcta and Rheum ribes. For this purpose, various plant parts—including the root bark, roots, fruits, and leaves of P. farcta, as well as the roots and stalks of R. ribes—were sequentially extracted employing n-hexane, ethyl acetate, and methanol. The α-glucosidase inhibition potential of the extracts was assessed. Subsequently, the phytochemical investigation was carried out on the ethyl acetate extract derived from the root of P. farcta, which exhibited the most pronounced inhibitory effect. This analysis resulted in the isolation and identification of six compounds, including four steroids, one pentacyclic triterpenoid, and one flavonoid. The isolated compounds underwent further evaluation for their α-glucosidase inhibition potential. Among them, daucosterol (3) demonstrated the highest potency, with an IC50 value of 18.6 µM.
subject: Original Articles
  topic: section
subject: Anaxagorea luzonensis A. Gray
  topic: keywords
subject: Annonaceae
  topic: keywords
subject: Antioxidant
  topic: keywords
subject: Analgesic
  topic: keywords
subject: Antipyretic
  topic: keywords
subject: Cytotoxic
  topic: keywords
subject: Phytoconstituents
  topic: keywords
subject: Spinasterol
  topic: keywords
creator: Sabiha Rahman
creator: Fatema-Tuz -Zohora
creator: Ramisa Anjum
creator: A.H.M. Nazmul Hasan
creator: Mohiuddin Ahmed Bhuiyan
creator: Choudhury Mahmood Hasan
creator: Mohammad Shahriar
rights: Copyright (c) 2025 Sabiha Rahman, Fatema-Tuz-Zohora, Ramisa Anjum, A.H.M Nazmul Hasan, Mohiuddin Ahmed Bhuiyan, Choudhury Mahmood Hasan, Mohammad Shahriar (Author) https://creativecommons.org/licenses/by/4.0
date: 2025-09-27
publication date: 2025-09-27
doi: 10.57647/tpr.2025.0902.12

In vitro assessment of the antihyperglycemic property of Prosopis farcta J. F. Macbr and Rheum ribes L. and phytochemical profiling of the most active extract
type: item
description: This study aimed to evaluate the α-glucosidase inhibition potential of Prosopis farcta and Rheum ribes. For this purpose, various plant parts—including the root bark, roots, fruits, and leaves of P. farcta, as well as the roots and stalks of R. ribes—were sequentially extracted employing n-hexane, ethyl acetate, and methanol. The α-glucosidase inhibition potential of the extracts was assessed. Subsequently, the phytochemical investigation was carried out on the ethyl acetate extract derived from the root of P. farcta, which exhibited the most pronounced inhibitory effect. This analysis resulted in the isolation and identification of six compounds, including four steroids, one pentacyclic triterpenoid, and one flavonoid. The isolated compounds underwent further evaluation for their α-glucosidase inhibition potential. Among them, daucosterol (3) demonstrated the highest potency, with an IC50 value of 18.6 µM.
subject: Original Articles
  topic: section
subject: α-Glucosidase
  topic: keywords
subject: Flavonoid
  topic: keywords
subject: Phytochemical analysis
  topic: keywords
subject: Prosopis farcta
  topic: keywords
subject: Rheum ribes
  topic: keywords
subject: Terpenoid
  topic: keywords
creator: Ismael Hasan Mohammed
creator: Vahed Zarial
creator: Mahdi Moridi Farimani
creator: Maryam Khratha
creator: Marzieh Tabefam
creator: Esmail Salih Ibrahim Kakey
rights: Copyright (c) 2025 Ismael Hasan Mohammed, Vahed Zarial, Mahdi Moridi Farimani, Maryam Khratha, Marzieh Tabefam, Esmail Salih Ibrahim Kakey (Author) https://creativecommons.org/licenses/by/4.0
date: 2025-09-27
publication date: 2025-09-27
doi: 10.57647/tpr.2025.0902.11

Phenolic composition and antioxidant capacity of leaves infusions of Annona muricata L. (Annnonaceae) from two regions of Mexico
type: item
description: Annona muricata L. (soursop) is a fruit of great economic importance and one of the plants most used in alternative medicine for its phytochemical composition. This work evaluates the phenolic composition and antioxidant capacity of infusions from Annona muricata leaves of three different ecotypes from two regions of Mexico. The infusions were characterized for total soluble phenolics, flavonoids, condensed tannins, total anthocyanins, and the antioxidant capacity was evaluated using different assays. The infusions of the analyzed ecotypes showed differences in polyphenol content and antioxidant activity. A positive relation between phenols and antioxidant capacity was observed for ABTS, FRAP, and ORAC. Epigallocatechin gallate (1.22-6.49 mg/g), rutin (3.47-8.73 mg/g), ellagic acid (5.25-14.13 mg/g), epicatechin (0.83-7.57 mg/g), and chlorogenic phenolic acids (4.42 mg/g) were quantified by HPLC. The infusion of leaves of the different soursop ecotypes showed differences in phenolic composition, antioxidant capacity and phenolic acids.
subject: Original Articles
  topic: section
subject: Bibliometric analysis
  topic: keywords
subject: Herbal medicine
  topic: keywords
subject: Murraya koenigii
  topic: keywords
subject: Scientific production
  topic: keywords
subject: Scopus database
  topic: keywords
subject: Rstudio
  topic: keywords
subject: VOSviewer
  topic: keywords
creator: Claudia Grijalva-Verdugo
creator: Salvador Horacio Guzmán-Maldonado
creator: César Leobardo Aguirre-Mancilla
creator: Carlos Alberto Núñez-Colin
creator: Juan Carlos Raya-Pérez
creator: Jesús Rubén Rodríguez-Núñez
rights: Copyright (c) 2025 Claudia Grijalva-Verdugo, Salvador Horacio Guzmán-Maldonado, César Leobardo Aguirre-Mancilla, Carlos Alberto Núñez-Colin, Juan Carlos Raya-Pérez, Jesús Rubén Rodríguez-Núñez (Author) https://creativecommons.org/licenses/by/4.0
date: 2025-09-27
publication date: 2025-09-27
doi: 10.57647/tpr.2025.0902.09

Green synthesis of iron nanoparticles via Rhizoclonum riparium (Roth) Harvey: A promising nanotherapy against the promastigote forms of Leishmania donovani parasites
type: item
description: Visceral leishmaniasis, a life-threatening parasitic disease caused by Leishmania donovani (Roth) Harvey and transmitted by Phlebotomus sandflies, continues to pose a significant global health concern. Conventional therapies like amphotericin B and miltefosine are often limited by severe side effects and the emergence of resistance, necessitating the development of safer and more effective alternatives. In this study, iron-nanoparticles (INPs) were biosynthesized using green macroalga Rhizoclonium riparium using FeCl₃ (0.005 M) at pH 6, and the synthesized INPs were characterized using UV-Vis spectroscopy, transmission electron microscopy (TEM), energy-dispersive X-ray (EDX) spectroscopy, and dynamic light scattering (DLS), confirming their spindle-shaped morphology with average particle size of 82 nm. In vitro anti-leishmanial activity of the INPs was assessed against L. donovani using MTT assay, demonstrating significant efficacy with an IC₅₀ value of 43.36 µg/mL. These results suggest that algal-mediated INPs represent a promising eco-friendly nanotherapeutic strategy for the treatment of visceral leishmaniasis.
subject: Original Articles
  topic: section
subject: Green nanosynthesis
  topic: keywords
subject: Iron nanoparticles (INPs)
  topic: keywords
subject: Leishmania donovani
  topic: keywords
subject: MTT assay
  topic: keywords
subject: Nanobiotechnology
  topic: keywords
subject: Rhizoclonium riparium (Roth) Harvey
  topic: keywords
subject: Visceral leishmaniasis
  topic: keywords
creator: Sagardeep Dey
creator: Anwesha Mondal
creator: Ishita Bhattacharya
creator: Nibedita Pyne
creator: Santanu Paul
rights: Copyright (c) 2025 Sagardeep Dey, Anwesha Mondal, Ishita Bhattacharya, Nibedita Pyne, Santanu Paul (Author) https://creativecommons.org/licenses/by/4.0
date: 2025-09-27
publication date: 2025-09-27
doi: 10.57647/tpr.2025.0902.08

Bibliometric analysis of Murraya koenigii (L.) Spreng research: Trends and hotspots
type: item
description: This paper aims to establish the significance of its studies within the current research landscape and to investigate their methodologies, conclusions, and trends. Utilizing the search term “Murraya koenigii,” we examined Scopus to identify bibliometric trends and hotspots. To accomplish this aim, 735 original publications published between 2013 and 2025 were retrieved and analyzed using bibliometric analysis, which was based on the Scopus Dataset results. BibTex and CVS (comma-separated values) files containing the data were exported, and Bibliometrix was implemented for the analysis. Studies on M. koenigii have been steadily increasing; 889 publications were identified in the Scopus database. Research articles accounted for 82.7% of the publications, followed by reviews (10.6%) and book chapters (3.1%). The findings presented here provide a preliminary visual summary of the scientific output of M. koenigii, taking into consideration the authors, study topics, journal citation coupling, and keyword analysis.
subject: Review Articles
  topic: section
subject: Bibliometric analysis
  topic: keywords
subject: Herbal medicine
  topic: keywords
subject: Murraya koenigii
  topic: keywords
subject: Scientific production
  topic: keywords
subject: Scopus database
  topic: keywords
subject: Rstudio
  topic: keywords
subject: VOSviewer
  topic: keywords
creator: Yuvianti Dwi Franyoto
creator: Arief Nurrochmad
creator: Nanang Fakhrudin
rights: Copyright (c) 2025 Yuvianti Dwi Franyoto, Arief Nurrochmad, Nanang Fakhrudin (Author) https://creativecommons.org/licenses/by/4.0
date: 2025-09-27
publication date: 2025-09-27
doi: 10.57647/tpr.2025.0902.07

Genotype × environment interaction, rhizome yield stability and selection for region specific stable genotypes in turmeric (Curcuma longa L.)
type: item
description: The present investigation was carried out to determine the stability over years among seventeen genetic stocks of turmeric assembled from different places in India for high and stable rhizomes yield. The differences among genetic stocks, years and g × e interactions were highly significant for rhizome yield. The stable genetic stocks were selected on the basis of stability parameters, high mean, regression coefficient (bi) around unity and mean square deviations from regression (s2di) near zero. The environmental indices for rhizome yield also indicated clear effects over the year’s i.e. 1.85 in year I followed by 9.95 in years II; -6.31 in year III and -5.29 in the year IV. The largest AMMI1 gain of the grand mean occurs over years for picking stable genotypes/clones. Further partitioning of genotypes on the basis of AMMI and Eberhart and Russell, (1966) model, the clones/genotypes T-8, T-12 and T-13 were highly stable; T-11 and T-14 adapted to the average stable in low yielding environment/year and T-9 ant T-17 genotypes/clones with below average stable suitable for high yielding environment were recommended for commercialization.
subject: Articles
  topic: section
subject: Curcumin
  topic: keywords
subject: stable clones
  topic: keywords
subject: multiplicative interaction
  topic: keywords
subject: Turmeric
  topic: keywords
subject: Anti-carcinogenic
  topic: keywords
creator: Raj Kishori Lal
creator: Sougata Sarkar
creator: Mohammad Zaim
rights: Copyright (c) 2024 Trends in Phytochemical Research
date: 2017-06-01
publication date: 2017-06-01
starting page: 93
ending page: 102

Phytochemical investigation and isolation of new compounds from the stems of Tinospora cordifolia Miers
type: item
description: Tinospora cordifolia (Willd.) Miers, (Menispermaceae), a glabrous, deciduous and trailing plant found in tropical India, China, Sri Lanka, Bangladesh and Myanmar. It is used to treat anemia, debility, diabetes, diarrhea, dysentery, dyspepsia, fevers, jaundice, rheumatism, urinary and skin diseases and snake bites. Its air-dried stem powder was exhaustively extracted with methanol. The concentrated methanolic extract was adsorbed on silica gel (60-120 mesh) for the preparation of a slurry. The dried slurry was chromatographed over silica gel column packed in petroleum ether. The column was eluted with petroleum ether, chloroform and methanol successively in order of increasing polarity to isolate a variety of new chemical constituents characterized as trans-cinnamoyl 2-n-hexanyl-7-methoxynaphthyl amide (2), trans-cinnamoyl 2-n- pentanyl- 6,7- dimethoxynaphthyl amide (3), trans-cinnamoyl 2-n-octanyl-7-methoxynaphthyl amide (4), β-D-arabinosyl-O-geranilan-10′-oate (5), 4,5,7-trimethoxy-2-naphthol-2-O-α-L- arabinofuranosyl-(2′→1′′)-O-α-L-arabinopyranosyl-2′′-O-pentane (6), 5,7-dimethoxy-2-naphthol-2-O-α-L-arabinopyranosyl-(2′→1′′)-α-L- arabinopyranosyl-2′′-O-decane (7) and 5-hydroxy-4''-methoxy-7- flavanoxy- (7→7'''')- β-O-labdan-1-en-3''''α,19''''-olide-18''''-oic acid (tinolabdenyl flavanone) (8) along with cetyl alcohol (1). The structures of all the isolated phytoconstituents have been established on the basis of spectral data analysis and chemical reactions.
subject: Articles
  topic: section
subject: chemical constituents
  topic: keywords
subject: stems
  topic: keywords
subject: Tinospora cordifolia
  topic: keywords
subject: isolation
  topic: keywords
subject: Characterization
  topic: keywords
creator: Shahnaj Sultana
creator: Mohammed Ali
creator: Mohammad Jameel
rights: Copyright (c) 2024 Trends in Phytochemical Research
date: 2017-06-01
publication date: 2017-06-01
starting page: 83
ending page: 92

Influence of extraction methods on total phenolic, flavonoids and antioxidant activity of Thymus kotchyanus L. extract in Semnan Province, Iran
type: item
description: Thymus kotchyanus L. is an aromatic plant belonging to Lamiaceae family and has promising traditional uses to treat some rural disorders. This species like other wild species of thyme is an aromatic plant and has been extensively used as an anti-inflammatory, expectorant, spasmolitic, sedative, antibacterial, antifungal, antioxidant and anti-infection agent. The present work concerned with ethnopharmacology as well as the influence of extraction methods on total phenolic contents (TPC), total flavonoids contents (TFC) and antioxidant activity of of Thymus kotchyanus L. extract in Semnan Province, Iran. In this regard, the aerial parts of plant were collected in the blooming period from Tash Mountain (2120 msl) in September 2015. Then, ethanol extracts of the plant were obtained by maceration and ultrasound‐assisted methods. In addition, TPC and TFC were determined through the standard spectrophotometric methods using the Folin-Ciocalteu and AlCl3 approaches, respectively. The antioxidant capacity was evaluated in vitro by the methods basing on 1,1-diphenyl-2-picryl hydrazyl radical scavenging (DPPH), total antioxidant capacity (TAC) and reducing power (RP) assays. The ultrasonic extract of plant was found to have higher amounts of TFC (81.17±1.07 mgQUE/g) and TPC contents (103.14±2.5 mgGAE/g) accounting for its high potential antioxidant activity (IC50=14.12±0.1 mg/mL) especially using the DPPH method. There was a strong positive correlation between influence of extraction methods on TPC, TFC and antioxidant activity of plant indicating that T. kotchyanus posess remarkable antioxidant activity and can be used potentially as a good source of natural antioxidant activities.
subject: Articles
  topic: section
creator: Masumeh Mazandarani
creator: Mohammad Ghafourian
rights: Copyright (c) 2024 Trends in Phytochemical Research
date: 2017-03-01
publication date: 2017-03-01
starting page: 33
ending page: 38

Extraction, purification, characterization and antioxidant activities of heat-resistance phenolic compounds from lemon pulp
type: item
description: Lemon as a citrus species is widely cultivated in the Mediterranean region, Turkey. The lemon pulp has been characterized as a rich source of phenolic and flavonoid compounds, phenolic acid as well as novel coumarin derivatives. It is usually obtained within the juice production in the food industries. In the current report, lemon pulp was initially dried and immediately after subjected to extraction with hot water and ethyl acetate. This study deals with the isolation of secondary metabolites along with determinations of 32 phenolic contents. After isolation of major components using Sephadex LH-20 column and silica gel, 11 phenolic components were totally characterized using the 1D and 2D NMR techniques. Lemon pulp known as citrus peel has been recognized as a potential source of coumarin, limonoids, and methoxylated flavones. The antioxidant activities of the limonin (LMN) and lemon pulp ethyl acetate extract (LPEA) were evaluated using in vitro methods involving total antioxidant activity, reducing power, inhibition of lipid peroxidation, scavenging activities of DPPH∙, H2O2 and ABTS+. and compared with standards at 10, 25, 50 and 100 μg.mL-1. According to the finding of this research, LMN and LPEA showed strong in vitro antioxidant activities and also were significantly exhibited an increase to be in a dose-dependent manner, (p <0.05).
subject: Articles
  topic: section
subject: characterization of natural compounds
  topic: keywords
subject: Antioxidant
  topic: keywords
subject: Lemon pulp
  topic: keywords
subject: isolation
  topic: keywords
creator: Yasar Ipek
creator: Tevfik Ozen
creator: Ibrahim Demirtas
rights: Copyright (c) 2024 Trends in Phytochemical Research
date: 2017-12-01
publication date: 2017-12-01
starting page: 199
ending page: 206

Isolation and identification of growth promoting endophytes from Artemisia annua L. and its effects on artemisinin content
type: item
description: Artemisinin, a sesquiterpene lactone, is a well-known antimalarial drug isolated from Artemisia annua L. (Asteraceae). Semi-synthetic derivatives of artemisinin like arteether, artemether, artesunate, etc. have also been explored for antimalarial as well as other pharmacological activities. Endophytes are microorganisms which reside inside the living tissues of host plants and can form symbiotic, parasitic or commensalistic relationship depending on the climatic conditions and host genotype. In this study, endophytic fungi were isolated from the leaves of A. annua and were identified using the conventional as well as molecular taxonomic methods. Endophytes were identified as: Colletotrichum gloeosporioides, Cochliobolus lunatus, Curvularia pallescens and Acremonium persicum. Growth promoting activity of endophytes has been reported earlier. After treating potted plants of A. annua with elicitor extracts prepared from these endophytic fungi, we observed an increase in the plant biomass as well as artemisinin content.
subject: Articles
  topic: section
subject: Cochliobolus lunatus
  topic: keywords
subject: elicitor
  topic: keywords
subject: Colletotrichum gloeosporioides
  topic: keywords
subject: Curvularia pallescens
  topic: keywords
subject: Acremonium persicum
  topic: keywords
creator: Mir Abid Hussain
creator: Vidushi Mahajan
creator: Irshad Ahmad Rather
creator: Praveen Awasthi
creator: Rekha Chouhan
creator: Prabhu Dutt
creator: Yash Pal Sharma
creator: Yashbir S. Bedi
creator: Sumit G. Gandhi
rights: Copyright (c) 2024 Trends in Phytochemical Research
date: 2017-12-01
publication date: 2017-12-01
starting page: 207
ending page: 214
doi: 10.71596/tpr.2024.3121448

Quantification of β-carotene, aucubin content, their associations and contribution to other economic traits in Plantago germplasm
type: item
description: Plant secondary metabolites have innumerable benefits in regard to human health. From the available 106 divergent lines of five Plantago species, a screening program was conducted to explore putative lines having best resources of aucubin and β-carotene. HPLC results indicated that seeds of selection LP3 of P. ovata contained highest (9.53%) and P. arenaria contained least (7.79%) β-carotene. Investigation was also conducted to assess the genetic variability, correlations and character contribution of β-carotene and aucubin content with other traits towards seed yield in a set of ten selected accessions in relation to seven traits in Plantago species. Results indicated that none of the accessions of any other species apart from P. lanceolata contained aucubin. PL-61 accession was detected to have maximum concentration (0.23%) of aucubin while the lowest (0.02%) was detected in PL-66. This opened the possibilities for nutritional value addition of β-carotene in seeds of isabgol.
subject: Articles
  topic: section
subject: Secondary metabolite
  topic: keywords
subject: Plantago sp
  topic: keywords
subject: Genetic advance
  topic: keywords
subject: HPLC
  topic: keywords
subject: Correlation
  topic: keywords
creator: Sougata Sarkar
creator: Raj Kishori Lal
creator: Ram Kishor Verma
rights: Copyright (c) 2024 Trends in Phytochemical Research
date: 2017-12-01
publication date: 2017-12-01
starting page: 215
ending page: 226