OICC Press Trends in Phytochemical Research 2588-3631 9 3 2025 09 30 Unveiling the potential antidiabetic, antidyslipidemic, and antimicrobial effects of methanolic root extracts of Nymphaea pubescens Willd. plant: Evidence from in-vitro and in-vivo approaches 181 190 10.57647/tpr.2025.0903.15 EN Sahid Ahmad Santo Department of Pharmacy, Noakhali Science and Technology University, Noakhali, Bangladesh https://orcid.org/0000-0002-9021-8008 Tanmay Deb Department of Pharmacy, Noakhali Science and Technology University, Noakhali, Bangladesh Md. Abdus Salam Department of Pharmacy, Noakhali Science and Technology University, Noakhali, Bangladesh https://orcid.org/0000-0003-1663-6786 Arafat Miah Department of Pharmacy, Noakhali Science and Technology University, Noakhali, Bangladesh Md. Saddam Hussain Department of Pharmacy, Noakhali Science and Technology University, Noakhali, Bangladesh https://orcid.org/0000-0002-8551-0039 Anamika Datta Department of Pharmacy, R. P. Shaha University, Narayanganj, Bangladesh Mohammad Nurul Amin Department of Pharmacy, Atish Dipankar University of Science and Technology, Dhaka, Bangladesh Mohammad Sarowar Uddin Department of Pharmacy, Noakhali Science and Technology University, Noakhali, Bangladesh Mohammad Safiqul Islam Department of Pharmacy, Noakhali Science and Technology University, Noakhali, Bangladesh Md. Shalahuddin Millat Department of Pharmacy, Noakhali Science and Technology University, Noakhali, Bangladesh Journal Article 2025 09 30 This study investigated the therapeutic potential of methanolic root extracts of Nymphaea pubescens Willd. with a focus on their antidiabetic, antilipidemic, and antimicrobial properties. The methanolic root extracts of the N. pubescens at higher doses (500 mg/kg) exhibited a pronounced serum blood glucose-lowering activity (p < 0.001) and also demonstrated remarkable outcomes in body weight loss (p < 0.01), closely resembling the effects of the standard metformin. The same experiment displayed a noticeable reduction in low-density lipoprotein cholesterol (LDL-C) and total cholesterol (TC) levels (p < 0.001), along with a notable elevation in high-density lipoprotein cholesterol (HDL-C) levels (p < 0.01). Furthermore, the plant extract at 500 mg/kg unveiled significant antimicrobial activities (p < 0.001) against both Staphylococcus aureus and Aspergillus niger and also exhibited significant (p < 0.01) inhibition of Escherichia coli. In summary, these findings highlight the multifaceted therapeutic potential of N. pubescens root extracts, warranting further investigation for their possible development into novel therapeutic agents.