OICC Press Trends in Phytochemical Research 2588-3631 9 4 2025 12 31 Integrated analysis of anatomy, phytochemistry, and biological potentials of Marrubium vulgare L. extracts 10.57647/tpr.2025.0904.23 EN Souad Djellali Department of Chemistry, Faculty of Sciences, University Setif 1 Ferhat Abbas, 19000, Setif, Algeria; Laboratory of Physical Chemistry of High Polymers, University Setif 1 Ferhat Abbas, Setif, Algeria https://orcid.org/0000-0002-0205-4649 Rachid Sahraoui Laboratory of Valorisation of Natural Biological Resources, University Setif 1 Ferhat Abbas, Setif, Algeria Yasmine Abdelouahed Department of Chemistry, Faculty of Sciences, University Setif 1 Ferhat Abbas, 19000, Setif, Algeria; Laboratory of Physical Chemistry of High Polymers, University Setif 1 Ferhat Abbas, Setif, Algeria Mohamed amine Benyahia Department of Matter Sciences, University Elbachir Elibrahimi, Bordj Bouarreridj, Algeria Mira Chribet Department of Matter Sciences, University Elbachir Elibrahimi, Bordj Bouarreridj, Algeria Journal Article 2025 12 31 This study reports on the phytochemical and bioactive characteristics of Marrubium vulgare L., harvested in northeast Algeria. After extraction using hydrodistillation and solvents with increasing polarity, 0.05% essential oil and 5.9% apolar crude extract were obtained. The plant presented a significant amount of phytochemicals like polyphenols (126.17 mg GAE/g), flavonoids (42.08 mg QE/g), tannins (45.07 mg TAE/g) which explained the high antioxidant power, evidenced by in vitro assays (FRAP, H2O2, OH• and cyclic voltammetry). Furthermore, the antimicrobial test against various microorganisms (Staphylococcus aureus, Salmonella sp, Shigella sp. Aspergillus niger, Aspergillus flavus) showed that the methanolic extract was broadly effective against all tested strains. However, among the less polar extracts, activity varied: essential oils showed moderate activity, dichloromethanic extract was active against most bacteria and petroleum ether extract showed significant antibacterial effects. These compelling results underscore the unique and significant therapeutic potential of the different extracts derived from Marrubium vulgare L.