OICC Press Trends in Phytochemical Research 2588-3631 9 1 (April 2025) 2025 03 30 From plant to nanoparticle: Evaluating the biological efficacy of Cannabis sativa L. methanol extract, unlocking the bioapotential of Cannabis sativa L. in the green fabrication of silver nanoparticles and their characterization 78 94 10.71596/tpr.2025.1203169 EN Majid Mohammadhosseini Department of Chemistry and Biochemistry, Sha.C., Islamic Azad University, Shahrood, Iran https://orcid.org/0000-0002-3188-4924 Behnam Mahdavi Department of Chemistry, Faculty of Science, Hakim Sabzevari University, Sabzevar, Iran Mohammad Reza Kianasab Department of Chemistry and Biochemistry, Sha.C., Islamic Azad University, Shahrood, Iran Journal Article 2025 03 30 Cannabis sativa L. (CSL) is a versatile plant recognized for its medicinal, industrial, and nutritional applications. Rich in bioactive compounds, it has been extensively studied for its phytochemical properties and biological activities. The current report focuses on the preparation of a methanol extract from the stems, flowers, and leaves of this plant, along with an assessment of its relevant biological activities, including antioxidant, antibacterial, and antifungal properties. In the second phase of this study, the green synthesis of Ag nanoparticles was successfully conducted using a methanolic extract of Cannabis sativa L. (MECS). In this process, CSL serves as a natural reducing and capping agent. The synthesized nanoparticles were subsequently characterized using Fourier-transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy (EDX), and transmission electron microscopy (TEM). This method offers a sustainable and environmentally-friendly approach to nanoparticle production.