OICC Press Trends in Phytochemical Research 2588-3631 8 1 2024 09 21 Blood coagulation effect of combined extract of Thymus vulgaris and Medicago sativa 73 79 10.71596/tpr.2024.4011452 EN Zahra Sadat Mashkani Department of Biology, Hakim Sabzevari University, Sabzevar, Iran Jafar Vatandoost Department of Biology, Hakim Sabzevari University, Sabzevar, Iran Toktam Hajjar Department of Biology, Hakim Sabzevari University, Sabzevar, Iran Behnam Mahdavi Department of Chemistry, Hakim Sabzevari University, Iran Journal Article 2024 09 21 Thymus vulgaris and Mediacgo sativa, are used as a traditional remedy in the treatment of bleeding disorders. Considering their probabilistic coagulation compounds, in an animal study, forty male mice were randomly divided into 5 groups (n = 8) as well as negative and positive control. Coagulation indices include bleeding time (BT), clotting time (CT), and the number of platelets (PLT) were examined on the 13th day of treatment. A significant reduction in the BT and CT tests, as well as a significant increase in PLT in the treated groups was observed. It is concluded that although the T. vulgaris and M. sativa extracts have a coagulation effect through primary homeostasis and a common pathway of secondary hemostasis, combined extracts are more effective than individual extracts. Moreover, phenolic and flavonoid compounds are the most affecting compounds that affect platelet number and aggregation.