OICC Press Trends in Phytochemical Research 2588-3631 4 2 2020 06 01 Antimicrobial efficacy and chemical constituents of pseudo-stem essential oils from Zingiber castaneum 93 100 EN Le Thi Huong School of Natural Science Education, Vinh University, 182 Le Duan, Vinh City, Nghệ An Province, Vietnam Trinh Thi Huong Graduate University of Science and Technology, Vietnam Vietnam Academy of Science and Technology, 18-Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam|Faculty of Natural Science, Hong Duc University, Thanh Hoa City, Thanh Hoa Province, Vietnam Nguyen Thi Bich School of Natural Science Education, Vinh University, 182 Le Duan, Vinh City, Nghệ An Province, Vietnam Nguyen Thi Viet School of Natural Science Education, Vinh University, 182 Le Duan, Vinh City, Nghệ An Province, Vietnam Abdulatif Olufemi Giwa-Ajeniya Department of Chemistry, Lagos State University, Lagos, Nigeria Isiaka Ogunwande Foresight Institute of Research and Translation, Nigeria Journal Article 2020 06 01 In this paper, chemical constituents and antimicrobial activity of essential oil from the pseudo-stem of Zingiber castaneum Škorničk. & Q.B. (Zingiberaceae) Nguyễn growing in Vietnam havw been reported. Essential oils were obtained by hydrodistillation using the Clevenger-type apparatus. Chemical components of the essential oil were analyzed by gas chromatography (GC) and gas chromatography coupled with mass spectrometry (GC/MS). The minimum inhibitory concentrations (MIC) were evaluated by the method of microdilution broth susceptibility assay. The main constituents of the oil were bicyclo germacrene (28, 15.8%), cis-β-elemene (18, 9.8%) and germacrene D (26, 9.2%). The pseudo-stem oil of Z. castaneum displayed antimicrobial activity against Pseudomonas aeruginosa (ATCC 25923), Aspergillus niger (ATCC 9763) and Fusarium oxysporum (ATCC 48112) with MIC values of 12.5 ± 0.57 μg/mL, 50 ± 1.00 μg/mL and 50 ± 0.50 μg/mL, respectively. The results indicate the potential of Z. castaneum essential oil as a source of antimicrobial agent.