OICC Press Trends in Phytochemical Research 2588-3631 2 1 2018 03 01 Phytochemicals in leaves and extracts of the variety “Plovdiv 7” of Bulgarian oriental tobacco (Nicotiana tabacum L.) 27 36 EN Venelina Popova University of Food Technologies, Department of Tobacco, Sugar, Vegetable and Essential Oils, Plovdiv, Bulgaria Tanya Ivanova University of Food Technologies, Department of Tobacco, Sugar, Vegetable and Essential Oils, Plovdiv, Bulgaria Albena Stoyanova University of Food Technologies, Department of Tobacco, Sugar, Vegetable and Essential Oils, Plovdiv, Bulgaria Vasil Georgiev Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, Bulgaria Tsveta Hristeva Tobacco and Tobacco Products Institute, Markovo, Bulgaria Violeta Nikolova Tobacco and Tobacco Products Institute, Markovo, Bulgaria Margarita Docheva Tobacco and Tobacco Products Institute, Markovo, Bulgaria Nikolay Nikolov Tobacco and Tobacco Products Institute, Markovo, Bulgaria Stanka Damianova Angel Kanchev University of Ruse, Razgrad Branch, Razgrad, Bulgaria Journal Article 2018 03 01 This study aims to identify and analyze the non-volatile fraction (chemicals, polyphenols, phenolic acids, flavonoids, and triterpenes) and volatile fraction (essential oil and crude extracted aroma fractions) present in the leaves of “Plovdiv 7” oriental tobacco variety (N. tabacum L.). The results show that the nicotine content in leaves reaches 2.3% (DW) and chemicals such as betulin (2340.7 g/g), carnosic acid (845.2 µg/g) and ursolic acid (596.0 µg/g) (595.95 g/g ), are also present. In terms of free phenolic acids, chlorogenic acid (2545.0 g/g), ferulic acid (1561.8 g/g), and vanillic acid (4461.9 g/g) are found to be dominant. The flavonoid profile is dominated by myricetin (134.2 g/g), quercetin (334.1 g/g), apigenin (493.0 g/g) and luteolin (445.6 g/g). In the volatile fraction, nineteen volatile components were identified (92.3%) where (E)-phytol (53.4%) was the major compound followed by solanone (6.8%), cis-5-butyl-4-methyldihydrofuran-2(3H)-one (6.4%) and dihydro-β-ionone (5.2%). In addition, the antimicrobial activity of “Plovdiv 7” tobacco leaf aroma extracts (concrete and resinoid extract) are tested against nine microbial strains. The results show a weak antimicrobial activity against Staphylococcus aureus and Bacillus subtilis bacteria, whereas no activity is recorded for the other seven microbial strains tested.