OICC Press Progress in Biomaterials 2194-0517 13 03 2024 09 30 Modulation of Optical Anisotropy in Soft Tissue Biomaterials Using Optical Clearing Agents: Implications for Structural Characterization and Biomedical Applications 10.57647/pibm.2024.132412 EN Saeed Ziaee Laser and Plasma Research Institute, Shahid Beheshti University, Tehran 19839 69411, Iran Mohammad Ali Ansari Laser and Plasma Research Institute, Shahid Beheshti University, Tehran 19839 69411, Iran Mehrdad Kalhori Laser and Plasma Research Institute, Shahid Beheshti University, Tehran 19839 69411, Iran Kamyab Hassani Laser and Plasma Research Institute, Shahid Beheshti University, Tehran 19839 69411, Iran Mohammad Hossein Naddaf Laser and Plasma Research Institute, Shahid Beheshti University, Tehran 19839 69411, Iran Valery V. Tuchin Institute of Physics and Science Medical Center, Saratov State University, 83 Astrakhanskaya str., Saratov 410012, Russia Journal Article 2024 09 30 This study systematically investigates the modulation of optical properties in anisotropic chicken skin tissue using five optical clearing  agents (OCAs): Sorbitol, Glycerol, Iohexol, Sucrose, and Glucose. Quantifying such changes is essential for the design of biomaterials  and the optimization of tissue-implant interfaces where laser propagation is critical. Utilizing goniometry and transmission  spectroscopy over a 60-minute period, our results consistently demonstrated that tissue optical clearing induced a significant increase in the scattering anisotropy factor (g), shifting from a baseline of g = 0.78−0.80 to post-clearing values reaching g = 0.96. This enhancement in forward scattering, accompanied by a concurrent decrease in the attenuation coefficient (µt), serves as a critical performance metric for  improving the Signal-to-Noise Ratio (SNR) in implanted optical sensors and ensuring efficient laser light delivery in photo-active scaffolds. Among the agents, Sorbitol exhibited the highest optical clearing efficacy, while simple sugars showed less pronounced effects. These findings provide a robust foundation for selecting optimal chemical agents to enhance laser light penetration depth for future embedded optical biosensors, directly benefiting the development of advanced biomaterials, and high-resolution imaging modalities such as Optical Coherence Tomography (OCT).