OICC Press Progress in Biomaterials 2194-0517 9 3 (September 2020) 2020 09 28 Artemisia annua L. as a promising medicinal plant for powerful wound healing applications 10.1007/s40204-020-00138-z EN Fatemeh Sadat Mirbehbahani Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, IR Fatemeh Hejazi Department of Advanced Technologies, Shiraz University, Shiraz, IR Najmeh Najmoddin Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, IR Azadeh Asefnejad Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, IR Journal Article 2020 09 28 Abstract Artemisia annua L. has been utilized for the first time in a nanofibrous wound dressing composition. The extract of this valuable plant provides anti-inflammatory, anti-bacterial and anti-microbial properties which can be considered as a promising medicinal component in therapeutic applications. In the present work, Artemisia annua L. was picked up from Gorgan forest area of Northern Iran and its extract was prepared by methanol as the extraction solvent. In the fabrication of wound dressing, Artemisia annua L. extract was mixed with gelatin and a nanofibrous structure was formed by electrospinning technique. To have a wound dressing with acceptable stability and optimum mechanical properties, this biologically active layer was formed on a PCL nanofibrous base layer. The fabricated double-layer wound dressing was analyzed chemically, structurally, mechanically and biologically. ATR-FTIR spectra of the prepared wound dressing contain functional groups of Artemisia annua L. as peroxide groups, etc. SEM micrographs of electrospun gelatin/ Artemisia annua L. confirmed the successful electrospinning process for producing Artemisia annua L.-containing nanofibers with mean diameter of 242.00 ± 67.53 nm. In vitro Artemisia annua L. release study of the fabricated wound dressings suggests a sustain release over 7 days for the crosslinked sample. In addition, evaluation of the in vitro structural stability of the prepared wound dressings confirmed the stability of the crosslinked nanofibrous structures in PBS solution environment. Biological study of the Artemisia annua L.-containing wound dressing revealed no cytotoxicity, good proliferation and attachment of the seeded fibroblasts cells and acceptable antibacterial property against Staphylococcus aureus bacteria.