OICC Press Progress in Biomaterials 2194-0517 11 3 (September 2022) 2022 07 25 Slow release curcumin-containing soy protein nanoparticles as anticancer agents for osteosarcoma: synthesis and characterization 10.1007/s40204-022-00197-4 EN Hadi Zare-Zardini Department of Biomedical Engineering, Meybod University, Meybod, IR Hematology and Oncology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, IR Medical Nanotechnology and Tissue Engineering Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi Hospital, Shahid Sadoughi University of Medical Sciences, Yazd, IR Hossein Soltaninejad Faculty of Interdisciplinary Science and Technology, Tarbiat Modares University, Tehran, IR Adel Ghorani-Azam Department of Forensic Medicine and Toxicology, School of Medicine, Urmia University of Medical Sciences, Urmia, IR Reza Nafisi-Moghadam Department of Radiology, Shahid Sadoughi University of Medical Sciences, Yazd, IR Navid Haddadzadegan Department of Anesthesiology and Critical Care, Shahid Sadoughi University of Medical Sciences, Yazd, IR Mojtaba Ansari Hematology and Oncology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, IR Seyed Houssein Saeed-Banadaki Department of Orthopedia, Shahid Sadoughi University of Medical Sciences, Yazd, IR Mohammad Reza Sobhan Department of Orthopedia, Shahid Sadoughi University of Medical Sciences, Yazd, IR Sima Mozafari Hematology and Oncology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, IR Mahlagha Zahedi Department of Pathology, Shahid Sadoughi University of Medical Sciences, Yazd, IR Journal Article 2022 07 25 Abstract Curcumin-containing soy protein nanoparticles (curcumin–SPNs) were synthesized by desolvation (coacervation) method and characterized by SEM, DLS, FTIR, and XRD. For anticancer evaluation, osteogenic sarcoma (SAOS2) cell lines were incubated with different concentrations of nanostructures. The dialysis method was used for assessment of drug release. Intracellular reactive oxygen species (ROS) were evaluated in IC50 dose after 24 h of exposure to free curcumin and curcumin–SPNs. Characterization data showed that the size of drug-free SPNs and curcumin–SPNs were 278.2 and 294.7 nm, respectively. The zeta potential of drug-free SPNs and curcumin–SPNs were − 37.1 and − 36.51 mv, respectively. There was no significant difference between the control and drug-free SPNs groups in terms of cell viability ( p  > 0.05). The viability of cells in different concentrations of the designed curcumin–SPNs in Saos2 cell line was significantly higher than free drug ( p