OICC Press Progress in Biomaterials 2194-0517 10 4 (December 2021) 2021 11 03 Stimuli-responsive electrospun nanofibers based on PNVCL-PVAc copolymer in biomedical applications 10.1007/s40204-021-00168-1 EN Sogand Safari Department of Polymer Processing, Iran Polymer and Petrochemical Institute, Tehran, IR Morteza Ehsani Department of Polymer Processing, Iran Polymer and Petrochemical Institute, Tehran, IR Mojgan Zandi Department of Biomaterials, Iran Polymer and Petrochemical Institute, Tehran, IR Journal Article 2021 11 03 Abstract Poly( N -vinylcaprolactam) (PNVCL) is a suitable alternative for biomedical applications due to its biocompatibility, biodegradability, non-toxicity, and showing phase transition at the human body temperature range. The purpose of this study was to synthesize a high molecular weight PNVCL-PVAc thermo-responsive copolymer with broad mass distribution suitable for electrospun nanofiber fabrication. The chemical structure of the synthesized materials was detected by FTIR and 1 HNMR spectroscopies. N -Vinyl caprolactam/vinyl acetate copolymers (159,680 molecular weight (g/mol) and 2.51 PDI) were synthesized by radical polymerization. The phase transition temperature of N -vinyl caprolactam/vinyl acetate copolymer was determined by conducting a contact angle test at various temperatures (25, 26, 28, and 30 ∘C\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^\circ{\rm C}$$\end{document} ). The biocompatibility of the nanofibers was also evaluated, and both qualitative and quantitative results showed that the growth and proliferation of 929L mouse fibroblast cells increased to 80% within 48 h. These results revealed that the synthesized nanofibers were biocompatible and not cytotoxic. The results confirmed that the synthesized copolymers have good characteristics for biomedical applications. Graphical abstract