OICC Press Journal of Theoretical and Applied Physics 2251-7235 18 4 2024 08 30 Preparing and Synthesizing Modified Electrospun Nanofibers via Online UV Method from Poly Acrylonitrile and Poly Vinyl Alcohol for Enzyme (glucose oxidase) Iimmobilization 10.57647/j.jtap.2024.1804.50 EN Sepideh Asadi Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran 0000-0003-0400-5071 Mehdi Jahanfar Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran 0000-0002-7247-2344 Saeed Javadi Anaghizi Central Laboratory of Shahid Beheshti University, Tehran, Iran 0000-0003-2801-1724 Dariush Minai-Tehrani Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran 0000-0003-3589-7324 Journal Article 2024 08 30 The study explores the use of nanofibers for enzyme immobilization, leveraging their high contact area and controlled cavities. By employing ultraviolet (UV) irradiation on a polymeric blend of polyvinyl alcohol (PVA) and poly acrylonitrile (PAN) during electrospinning, nanofibers were fabricated for glucose oxidase (GOD) immobilization. UV treatment enhanced carboxyl group presence on nanofiber surfaces, as confirmed by Fourier transform infrared (FTIR) analysis. Enzyme activity assessments demonstrated a 33% increase in immobilized enzyme activity when PVA content in the nanofibers was raised from 1% to 3%. Scanning electron microscopy (SEM) images revealed that UV treatment helped maintain nano filament structures post-immobilization, aiding enzyme retention and controlled release. Atomic force microscopy (AFM) images displayed increased roughness in UV-treated nanofibers. The study showcased GOD reusability up to 12 cycles in 3% PVA-containing samples.