OICC Press Journal of Theoretical and Applied Physics 2251-7235 20 5 2026 10 31 Investigation of Renal Failure Impact on Blood Mineral Equilibrium Employing Laser Spectroscopy 10.57647/jtap.2026.2005.06 EN Noor Hameed Nasif Department of Physics, College of Science, University of Diyala, Diyala, Iraq Bahaa Jawad Alwan Department of Physics, College of Science, Mustansiriyah University, Baghdad, Iraq Nedaa Monther Salman Open Educational college, Baghdad, Iraq Sarah Mahmoud Abdallh Department of Physics, College of Science, University of Diyala, Diyala, Iraq Journal Article 2026 10 31 Patients suffering from renal failure often exhibit severe disturbances of mineral and electrolyte homeostasis, placing a heavy demand on rapid & accurate diagnostic techniques for routine clinical monitoring. In this pilot study, we report the successful application of Laser-Induced Breakdown Spectroscopy (LIBS) to examine hemodialysis effects on blood mineral balance in serum samples from healthy subjects and renal failure patients prior to and post-dialyzer. We proposed a new relative intensity-based spectral method, termed the Intensity Ratio Index (IRI), to analyze differences of calcium (Ca), potassium (K), phosphorus (P), magnesium (Mg), and zinc (Zn) without complex calibration processes. The main contribution of this work is to demonstrate the viability of LIBS in a fast, less invasive, and cost-effective manner for assessing dialysis efficacy as well as mineral imbalance online. The resulted values were highly different for potassium and phosphorus in pre-dialysis, with effect ratios of around 73.8% & 74.2%, respectively; these parameters suggest serious electrolyte disturbance associated with renal dysfunction. In contrast, post-dialysis analysis presented partial restoration of mineral homeostasis exclusively for calcium with an improvement ratio around 36%. Additionally, the study validated that LIBS can detect rapid and sensitive relative spectral changes in both major and trace elements. These results suggest great potential for the use of LIBS as an effective clinical diagnostic method for monitoring renal failure and determining hemodialysis effectiveness.