OICC Press Journal of Theoretical and Applied Physics 2251-7235 19 4 2025 08 31 Impact of non-thermal plasma on radish seeds using plasma jet at atmospheric pressure 10.57647/j.jtap.2025.1904.37 EN Bablu Kant Thakur Department of Physics, Trichandra Multiple Campus, Tribhuvan University, Kathmandu, Nepal Rajendra Shrestha Department of Physics, Nepal Banepa Polytechnic Institute, Banepa, Kavre, Neap; Department of Physics, Patan Multiple Campus, Tribhuvan University, Lalitpur, Nepal Arun Kumar Shah Department of Physics, Patan Multiple Campus, Tribhuvan University, Lalitpur, Nepal Ram Lal Shah Department of Physics, Padmakanya Multiple Campus, Tribhuvan University, Kathmandu, Nepal Reeta Silpakar Department of Information Technology, Nepal Banepa Polytechnic Institute, Banepa, Kavre, Neapl Lekha Nath Mishra Department of Physics, Nepal Banepa Polytechnic Institute, Banepa, Kavre, Neapl Journal Article 2025 08 31 Using a plasma jet at ambient temperature and atmospheric pressure, the investigation was conducted to determine the potential effects of non-thermal plasma on radish seeds. A cold atmospheric plasma jet with an argon gas source was employed for this system. Six treatment dose and one control were utilized to measure the germination parameters. The impact of atmospheric pressure plasma on treated radish seed germination over several time periods was observed under a working voltage 5 kV, and an argon flow rate 2 lmin-1. The germination percentage, and shoot length were all boosted at an ambient condition. The optimal germination appears to be two minutes of operating time whereas it reduces for 2.5 and 3 minutes. Both untreated and plasma-treated seeds were characterized using Fourier Transform Infrared Spectroscopy. Radish leaves' chlorophyll content was measured and it is revealed that the treated seeds have more chlorophyll. Electrical and optical techniques have been used to create and characterize the plasma jet.