OICC Press Journal of Nanostructure in Chemistry 2193-8865 3 1 (December 2013) 2013 04 23 Size-dependent in vivo/in vitro results of homoeopathic herbal extracts 10.1186/2193-8865-3-18 EN Rajesh Barve Department of Repertory, Virar Homoeopathic Medical College, Virar, Maharashtra, 401305, IN Ramesh Chaughule Department of Chemistry, Ramnarain Ruia College, Matunga, Mumbai, 400019, IN Journal Article 2013 04 23 Abstract This paper presents evidence-based results of clinical trial on the use of nanosized particles in homoeopathic Terminalia arjuna tincture. Dynamic light scattering technique demonstrates that homoeopathic tincture is a mixture of particles varying from micron to nanometre sizes. The process of succussion, homogenization and filtration brings down the particles of source material to nanosize as a ‘top-down’ process. As compared to the conventionally used micron-sized T. arjuna powder, the results show significant clinical improvement due to the presence of nanoparticles in tincture by the above method. Similar results were reconfirmed on experiments with bacterial cultures. From clinical trials, it has been confirmed that the dose of medicine required by the patient is significantly reduced with the reduction in particle size. No toxicity was observed even after giving medicine for more than 8 weeks. Size-dependent sensitivity of the drug can help to tackle the problem of drug resistance in bacteria.