OICC Press Journal of Nanostructure in Chemistry 2193-8865 4 3 (September 2014) 2014 06 05 Anti-bacterial activities of silver nanoparticles synthesized from plant leaf extract of Abutilon indicum (L.) Sweet 10.1007/s40097-014-0106-1 EN M. Prathap Department of Plant Science, Bharathidasan University, Tiruchirappalli, Tamil Nadu, 620024, IN A. Alagesan Department of Plant Science, Bharathidasan University, Tiruchirappalli, Tamil Nadu, 620024, IN B. D. Ranjitha Kumari Department of Plant Science, Bharathidasan University, Tiruchirappalli, Tamil Nadu, 620024, IN Journal Article 2014 06 05 Abstract Abutilon indicum (L.) Sweet is an Asian phytomedicinal plant and it has hypoglycemic and anti-bacterial activities. The nano silver was successfully synthesized from leaf extract using A. indicum (L.) Sweet. Nitrate reductase enzyme or other extra cellular proteins released from leaf extract involve in reduction of nitrate and formation of silver ions occurs. These proteins or enzymes act as template for the silver nucleation sites for the formation of silver nanocrystals and also act as capping agents (peptides or proteins) which control the size and shape of silver nanoparticles. FT-IR reveals that protein molecules can be bound to silver nanoparticles and XRD result confirmed crystal structure of nanoparticles. On the basis of our results, extract of A. indicum (L.) Sweet leaf protein molecules plays a vital role in reducing and capping of silver nanoparticles at 50 μl concentrations which act as anti-bacterial agent to control pathogenic microorganisms of Klebsiella pneumonia , Salmonella typhi , Bacillus subtilis , and Proteus vulgaris .