OICC Press Journal of Nanostructure in Chemistry 2193-8865 9 2 (June 2019) 2019 04 29 Biosynthesis, characterization and antimicrobial activity of gold nanoparticles from leaf extracts of Annona muricata 10.1007/s40097-019-0301-1 EN Aderonke Folorunso Department of Chemistry, Louisiana State University, Louisiana, US Sunday Akintelu Department of Pure and Applied Chemistry, Ladoke Akintola University of Technology, Ogbomoso, NG Abel Kolawole Oyebamiji Department of Pure and Applied Chemistry, Ladoke Akintola University of Technology, Ogbomoso, NG Department of Basic Sciences, Adeleke University, Ede, Osun State, NG Samuel Ajayi Department of Chemistry, Covenant University, Ota, NG Babawale Abiola Department of Pure and Applied Chemistry, Ladoke Akintola University of Technology, Ogbomoso, NG Ibrahim Abdusalam Department of Chemical Sciences, Fountain University, Osogbo, NG Adetoun Morakinyo Department of Biochemistry, Adeleke University, Ede, NG Journal Article 2019 04 29 Abstract The need to divulge the rapid synthesis, non-hazardous, cost effectiveness and eco-friendly methods for the synthesis of nanoparticles utilizing plants is of great importance. This is as a result of high toxicity associated with the chemical method of synthesizing nanoparticles. The aim of this study was to investigate the potency of the synthesized gold nanoparticle against selected human pathogens. Gold nanoparticles were synthesized by reacting 1 mM gold chloride solution with leaf extract of Annona muricata . The synthesized gold nanoparticles were characterized with UV–visible spectrophotometer, transmission electron microscope (TEM) and Fourier transformed infrared spectroscopy (FTIR). The antibacterial and antifungal activities of the synthesized gold nanoparticles were also investigated. The morphology, size, and structural properties of synthesized gold nanoparticles were determined with TEM analysis which showed spherical mono-dispersed structure with an average particle size of 25.5 nm. FTIR analysis reveal band at 3271.14, 2111.91 and 1637.82 cm −1 corresponding to –N–H, –C=C, and –C–N functional groups that are responsible for the capping and stabilization of synthesized gold nanoparticles. The effectiveness of the gold nanoparticle against the test pathogens increases as the concentration of gold nanoparticle increases. The percentage of zones of inhibition of synthesized gold nanoparticle against test fungi and bacteria ranges from 30 to 66% and 40 to 54%, respectively. The potency of the synthesized gold nanoparticle against the selected fungi and bacteria increases with increase in concentration of gold nanoparticle. Therefore, the antibacterial and antifungal investigation revealed that the synthesized gold nanoparticles exhibited good antimicrobial activity.