OICC Press Journal of Nanostructure in Chemistry 2193-8865 9 1 (March 2019) 2018 12 04 Green synthesis of silver nanoparticles using the plant extract of Salvia spinosa grown in vitro and their antibacterial activity assessment 10.1007/s40097-018-0291-4 EN Saba Pirtarighat Department of Biotechnology, Imam Khomeini International University (IKIU), Qazvin, IR Maryam Ghannadnia Department of Horticultural Science, Imam Khomeini International University (IKIU), Qazvin, IR Saeid Baghshahi Department of Materials Science and Engineering, Imam Khomeini International University (IKIU), Qazvin, IR Journal Article 2018 12 04 Abstract Researchers use bionanotechnology techniques as eco-friendly and cost-effective routes to fabricate nanoparticles and nanomaterials. The present study confirms the ability of plant extract of Salvia spinosa grown under in vitro condition for the biosynthesis of silver nanoparticles (Ag NPs) for the first time. The surface plasmon resonance found at 450 nm confirmed the formation of Ag NPs. Moreover, FESEM images showed that nanoparticles had spherical morphology. Furthermore, XRD analysis confirmed the crystalline nature of the particles. FTIR analysis was carried out to identify possible biomolecules responsible in bioreduction of silver ions. Antimicrobial assay verified bactericidal activity of biosynthesized Ag NPs against Gram-positive and Gram-negative bacteria. According to the results, by growing the plants under controlled conditions, it is feasible to synthesize nanoparticles with desired properties. Graphical abstract