OICC Press Journal of Nanostructure in Chemistry 2193-8865 8 1 (March 2018) 2017 12 14 Aflatoxin M1 detoxification from infected milk using Fe3O4 nanoparticles attached to specific aptamer 10.1007/s40097-017-0250-5 EN Fatemeh Javani Jouni Department of Biophysics, Faculty of Biological Science, Tarbiat Modares University (TMU), Tehran, IR Jaber Zafari Department of Toxicology, Ahvaz Jundishapur University of Medical, Ahvaz, IR Parviz Abdolmaleki Department of Biophysics, Faculty of Biological Science, Tarbiat Modares University (TMU), Tehran, IR Hossein Vazini Nursing Department, Hamedan Branch, Islamic Azad University, Hamedan, IR Leila Ghandi Chemistry Department, Tarbiat Modares University (TMU), Tehran, IR Mohamad Satari Department of Biophysics, Faculty of Biological Science, Tarbiat Modares University (TMU), Tehran, IR Journal Article 2017 12 14 Abstract Aflatoxins are a kind of mycotoxins that are mostly produced by a group of molds such as Aspergillus flavus and Aspergillus parasiticus . The studies on different parts of Iran showed that AFM 1 infection of milk is higher than European Union’s standard. In this study, the specific aptamer with the carboxylic group at the end of 5′ and Fe 3 O 4 nanoparticles with amine groups was synthesized. Morphological and structural qualities of Fe 3 O 4 were determined by the Fourier-transform infrared spectrograph (FTIR), dynamic light scattering, scanning electron microscope, and X-ray diffraction devices. The specificity of the aptamer to AFM 1 was investigated in adjacent of AFM 1 and aflatoxin B 1 . Then, aptamers were attached to nanoparticles to improve synthetic qualities and to ease of its detachment. The attachment was approved by FTIR method. The complex (Fe 3 O 4 –APT) was then added to infected milk and after the proper time was detached from the milk using a magnet. The remained amount of AFM 1 was attained in milk using high-performance liquid chromatography. Our result showed that this method for aflatoxin detoxification is much more effective than conventional methods based on recognition of AFM 1 and their concentration in infected milk. This method is more applicable, faster, and cheaper highlighting its application in milk factories. Graphical abstract