OICC Press Journal of Nanostructure in Chemistry 2193-8865 7 4 (December 2017) 2017 11 30 Preparation of human serum albumin nanoparticles using a chemometric technique 10.1007/s40097-017-0242-5 EN Nastaran Hosseinifar Department of Applied Chemistry, Faculty of Chemistry, Islamic Azad University, Tehran-North Branch, Tehran, IR Amir Abdolah Mehrdad Sharif Department of Applied Chemistry, Faculty of Chemistry, Islamic Azad University, Tehran-North Branch, Tehran, IR Department of Analytical Chemistry, Faculty of Chemistry, Islamic Azad University, North Tehran Branch, Tehran, IR Navid Goodarzi Nanotechnology Research Centre, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, IR Mohsen Amini Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, IR Rassoul Dinarvand Nanotechnology Research Centre, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, IR Journal Article 2017 11 30 Abstract Human serum albumin (HSA), a versatile protein carrier for drug delivery, is an ideal material to fabricate nanoparticles for drug delivery systems. These nanoparticles can accumulate in tumor interstitium due to the enhanced permeability and retention effect. The most important characteristics of HSA nanoparticles are particle size, shape, and zeta potential. A chemometric approach was applied for HSA nanoparticles’ size optimization in this study. The effects of three experimental parameters; pressure ( P ) or power, organic solvent volume ( V ), and time ( T ), were investigated under sonication and high-pressure homogenization, using multivariate analysis. The trials were performed based on the Box–Behnken experimental design. The criteria for the appraisal of the descriptive ability of a multinomial were R 2  = 0.819, standard error = 20.420, and F -ratio = 19.550. The method was optimized with respect to the nanoparticles’ size as a response. The Box–Behnken experimental design was applied to optimize and trial the robustness of the HSA nanoparticle preparation method.