OICC Press Journal of Nanostructure in Chemistry 2193-8865 14 3 2022 09 30 Thermoresponsive and antibacterial two-dimensional polyglycerol-interlocked-polynipam for targeted drug delivery 10.1007/s40097-022-00514-0 EN Nasim Khosravani Department of Chemistry, Faculty of Science, Lorestan University, Khoramabad, 68151-44316, IR Vahid Ahmadi Institut für Chemie und Biochemie, Freie Universität Berlin, Berlin, 14195, DE Ali Kakanejadifard Department of Chemistry, Faculty of Science, Lorestan University, Khoramabad, 68151-44316, IR Mohsen Adeli Department of Chemistry, Faculty of Science, Lorestan University, Khoramabad, 68151-44316, IR Journal Article 2022 09 30 Abstract Two-dimensional polymeric networks are a new class of polymers with interesting physicochemical and biological properties. They promise a wide range of future biomedical applications including pathogen interactions, drug delivery, bioimaging, photothermal, and photodynamic therapy, owing to their unique features, such as high surface area and multivalent interactions at nano-biointerfaces. In this work, a thermosensitive two-dimensional polymeric network consisting poly( N -isopropylacrylamide) (pNIPAM) chains that are mechanically interlocked by a polyglycerol platform was synthesized and used for bacteria incapacitation. Two-dimensional hyperbranched polyglycerol (2D-hPG) was synthesized by a graphene-assisted strategy and used for encapsulation of azobisisobutyronitrile (AIBN). Radical polymerization of N -isopropylacrylamide by encapsulated AIBN resulted in thermoresponsive platforms with ~ 500 nm lateral size and 20–50 nm thickness. Due to its porous structure, 2D-PNPG was able to efficiently load antibiotics, such as tetracycline (TC) and amoxicillin (AMX). The rate of release of antibiotics from 2D-PNPG and the antibacterial activity of the system correlated with the variation of temperature as a result of the thermosensitivity of 2D-PNPG. This study shows that two-dimensional polymers are efficient platforms for future biomedical applications including drug delivery and bacteria incapacitation. Graphical abstract Thermoresponsive two-dimensional nanomaterials with the ability of loading therapeutic agents and antibacterial activity are synthesized and characterized.