OICC Press Journal of Nanostructure in Chemistry 2193-8865 13 6 (December 2023) 2022 03 15 Highly sensitive electrochemical azaperone sensor based on magnetic silica –NH2-CS2 in the ostrich meat and rat plasma and its comparison with HPLC–MS/MS 10.1007/s40097-022-00488-z EN Faezeh Gandomi Students’ Scientific Research Center (SSRC), Tehran University of Medical Sciences (TUMS), Tehran, IR Mohammad Javad Taghizadeh Department of Chemistry, Faculty of Science, University of Imam Hossein, Tehran, IR Elnaz Marzi Khosrowshahi Food and Drug Safety Research Center, Tabriz University of Medical Sciences, Tabriz, IR Maryam Saleh Mohammadnia Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, IR Neda Irannejad Department of Chemistry, Isfahan University of Technology, Esfahān, 84156–83111, IR Esmail Sohouli Department of Chemistry, Faculty of Science, University of Imam Hossein, Tehran, IR Hermann Ehrlich Institute of Electronic and Sensor Materials, TU Bergakademie Freiberg, Freiberg, 09599, DE Center for Advanced Technology, Adam Mickiewicz University, Poznan, 61614, PL Yvonne Joseph Institute of Electronic and Sensor Materials, TU Bergakademie Freiberg, Freiberg, 09599, DE Mehdi Rahimi-Nasrabadi Institute of Electronic and Sensor Materials, TU Bergakademie Freiberg, Freiberg, 09599, DE Chemical Injuries Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, IR Faculty of Pharmacy, Baqiyatallah University of Medical Sciences, Tehran, IR Journal Article 2022 03 15 Abstract Azaperone is a very important phenylbutanone-based neurotransmitter used in the treatment of some animal (veterinary) clinics. This compound has various nerve and tendon stabilizing agents on livestock and animals. Muscular injection of azaperone is used to reduce stress in livestock and reduce their acting. In the present work, Fe 3 O 4 @SiO 2 -NH 2 /CS 2 nanocomposite was synthesized and thoroughly characterized using FE-SEM, FT-IR, and XRD technique. The glassy carbon electrode was then modified with nanocomposite to fabricate a sensor named GCE/Fe 3 O 4 @SiO 2 -NH 2 /CS 2 . The application of modified electrode was tested for analysis of azaperone in ostrich meat and rat plasma. The electrochemical behavior of azaperone was tested using differential pulse and cyclic voltammetry. In Briton–Robinson buffer solution (pH = 6), azaperone had an oxidation peak at 0.82 V. Cyclic voltammetry studies indicated that the azaprone oxidation process on the modified electrode is irreversible. Experimental variables, including pH and accumulation time were optimized by monitoring the cyclic voltammetry responses toward azaperone peak current. Measurement of azaperone by differential pulse voltammetry technique showed linearity of anodic peak current vs. azaperone concentration in a range of 0.01–100.0 μM with detection and quantification limits of 3 nM and 10 nM, respectively. Also, the effect of disturbance of some species as possible interferers on the electrochemical response of azaperone was checked out. Finally, the capability of the fabricated sensor for azaperone measurement was successfully tested in ostrich meat and rat plasma as real samples. Graphical abstract