OICC Press Journal of Nanostructure in Chemistry 2193-8865 12 5 (October 2022) 2022 02 14 Exploring nano-enabled CRISPR-Cas-powered strategies for efficient diagnostics and treatment of infectious diseases 10.1007/s40097-022-00472-7 EN Ankit Kumar Dubey Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, 600036, Chennai, Tamil Nadu, IN Vijai Kumar Gupta Biorefining and Advanced Materials Research Center, Scotland’s Rural College (SRUC), Edinburgh, EH9 3JG, GB Małgorzata Kujawska Department of Toxicology, Poznan University of Medical Sciences, Poznań, 60-631, PL Gorka Orive NanoBioCel Group, Laboratory of Pharmaceutics, School of Pharmacy, University of the Basque Country UPV/EHU, Vitoria-Gasteiz, ES CIBER Bioengineering, Biomaterials and Nanomedicine (CIBERBBN), Institute of Health Carlos III, Madrid, ES Bioaraba Health Research Institute, Nanobiocel Research Group, Vitoria-Gasteiz, ES University Institute for Regenerative Medicine and Oral Implantology, UIRMI (UPV/EHU-Fundación Eduardo Anitua), Vitoria-Gasteiz, ES Singapore Eye Research Institute, Singapore, SG Nam-Young Kim Department of Electronics Engineering, RFIC Bio Centre, NDAC Centre, RFIC Bio Centre, NDAC Centre, Kwangwoon University, Seoul, 01897, KR Chen-zhong Li Center for Cellular and Molecular Diagnostics, Tulane University School of Medicine, New Orleans, LA, 70112, US Department of Biochemistry and Molecular Biology, Tulane University School of Medicine, New Orleans, LA, 70112, US Yogendra Kumar Mishra Mads Clausen Institute, NanoSYD, University of Southern Denmark, Sønderborg, 6400, DK Ajeet Kaushik NanoBioTech Laboratory, Health System Engineering, Department of Natural Sciences, Florida Polytechnic University, Lakeland, FL-33805, US Journal Article 2022 02 14 Abstract Biomedical researchers have subsequently been inspired the development of new approaches for precisely changing an organism’s genomic DNA in order to investigate customized diagnostics and therapeutics utilizing genetic engineering techniques. Clustered Regulatory Interspaced Short Palindromic Repeats (CRISPR) is one such technique that has emerged as a safe, targeted, and effective pharmaceutical treatment against a wide range of disease-causing organisms, including bacteria, fungi, parasites, and viruses, as well as genetic abnormalities. The recent discovery of very flexible engineered nucleic acid binding proteins has changed the scientific area of genome editing in a revolutionary way. Since current genetic engineering technique relies on viral vectors, issues about immunogenicity, insertional oncogenesis, retention, and targeted delivery remain unanswered. The use of nanotechnology has the potential to improve the safety and efficacy of CRISPR/Cas9 component distribution by employing tailored polymeric nanoparticles. The combination of two (CRISPR/Cas9 and nanotechnology) offers the potential to open new therapeutic paths. Considering the benefits, demand, and constraints, the goal of this research is to acquire more about the biology of CRISPR technology, as well as aspects of selective and effective diagnostics and therapies for infectious illnesses and other metabolic disorders. This review advocated combining nanomedicine (nanomedicine) with a CRISPR/Cas enabled sensing system to perform early-stage diagnostics and selective therapy of specific infectious disorders. Such a Nano-CRISPR-powered nanomedicine and sensing system would allow for successful infectious illness control, even on a personal level. This comprehensive study also discusses the current obstacles and potential of the predicted technology. Graphical abstract