10.1007/s40097-021-00460-3

Comparative evaluation of chemically and green synthesized zinc oxide nanoparticles: their in vitro antioxidant, antimicrobial, cytotoxic and anticancer potential towards HepG2 cell line

  • Hajra Ashraf1,
  • Bisma Meer1,
  • Junaid Iqbal1,
  • Joham Sarfraz Ali1,
  • Anisa Andleeb1,
  • Hira Butt2,
  • Muhammad Zia1,
  • Azra Mehmood2,
  • Muhammad Nadeem3,
  • Samantha Drouet4,
  • Jean-Philippe Blondeau5,
  • Nathalie Giglioli-Guivarc’h6,
  • Chunzhao Liu7,
  • Christophe Hano4,
  • Bilal Haider Abbasi*1,
  1. Department of Biotechnology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320, PK
  2. National Centre of Excellence in Molecular Biology, University of Punjab, Lahore, PK
  3. Department of Biotechnology, Institute of Biotechnology, CECOS University, Peshawar, 25100, PK
  4. Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), INRA USC1328, Université d’Orléans, Orléans Cedex 2, 45067, FR
  5. Conditions Extrêmes et Matériaux: Haute Température et Irradiation (CEMHTI) CNRS UPR3079, Orléans, 45071, FR
  6. EA2106 Biomolecules et Biotechnologies Vegetales, Universite Francois-Rabelais de Tours, Tours, FR
  7. State Key Laboratory of Bio-Fibers and Eco-Textiles, Institute of Biochemical Engineering, Affiliated Qingdao Central Hospital, College of Materials Science and Engineering, Qingdao University, Qingdao, 266071, CN

Published in Issue 04-01-2022

How to Cite

Ashraf, H., Meer, B., Iqbal, J., Ali, J. S., Andleeb, A., Butt, H., Zia, M., Mehmood, A., Nadeem, M., Drouet, S., Blondeau, J.-P., Giglioli-Guivarc’h, N., Liu, C., Hano, C., & Abbasi, B. H. (2022). Comparative evaluation of chemically and green synthesized zinc oxide nanoparticles: their in vitro antioxidant, antimicrobial, cytotoxic and anticancer potential towards HepG2 cell line. Journal of Nanostructure in Chemistry, 13(2 (April 2023). https://doi.org/10.1007/s40097-021-00460-3
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Abstract

Abstract The major goal of this study was to fabricate ZnO nanoparticles (NPs) via chemical and biological routes to evaluate and compare their physiochemical behavior (size and morphology) and biological potentials. The synthesized NPs were confirmed via various spectroscopy and imagining techniques such as XRD, FT-IR, HPLC and SEM. The characterized NPs were then assessed for various in vitro biological applications. Furthermore, apoptotic potential was investigated using HepG2 cell lines by evaluating various signature markers of apoptosis including mitochondrial membrane potential, reactive oxygen/nitrogen (ROS/RNS) production, peroxidases and pro-apoptotic caspase 3 activation. Crystalline, hexagonal structured NPs with an average crystalline size distribution of 32 nm and 23 nm was obtained. Both green-mediated zinc oxide nanoparticles (G-ZnO NPs) and chemically derived NPs (C-ZnO NPs) exhibited high antioxidant, moderate enzyme inhibition, antibacterial and cytotoxicity potential. However, G-ZnO NPs exhibited excellent DPPH (80.1% ± 1.3%), TPC (97 ± 1.22 µgAAE/mg), TRP (94.5 ± 1.48 µgAAE/mg), lipase inhibition (82%), urease inhibition (81.3%), and α-amylase inhibition (18.9%) activity as compared to C-ZnO NPs. Both G-ZnO NPs and C-ZnO NPs showed good antimicrobial potential, however, effect of G-ZnO NPs was more potent than counter C-ZnO NPs. Results from apoptotic assays revealed that G-ZnO NPs showed excellent apoptotic potential in contrast to C-ZnO NPs. Overall results suggested that green route-mediated ZnO NPs exhibits excellent biological potential and could be used for future biomedical applications especially in antimicrobial and cancer therapeutics. To the best of our knowledge, this is the first ever study on Boerhavia diffusa linn -mediated biosynthesis of ZnO nanoparticles and evaluation of their biological activities. Graphical abstract

Keywords

  • Zinc oxide nanoparticles,
  • Chemical synthesis,
  • Green synthesis,
  • Characterization,
  • Phytochemical,
  • Antioxidant,
  • Anti-bacterial,
  • Cell viability

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