@article{Varaei_Rahimian_Askari_Bahador_Pourhajibagher_Aminian_2024, title={Pre industrialization assessment of polyclonal IgY antibodies to whole-cell S. mutans}, volume={15}, url={https://oiccpress.com/international-journal-of-industrial-chemistry/article/pre-industrialization-assessment-of-polyclonal-igy-antibodies-to-whole-cell-s-mutans/}, DOI={10.57647/j.ijic.2024.1502.15}, abstractNote={Dental caries is a multifactorial disease with high prevalence around the world. Various strategies have been developed and proved beneficial in managing the  disease; however, novel techniques have also been investigated. One of such strategies involves the administration of polyclonal antibodies (passive immunization), usually in the form of an oral mouthwash. While many studies have taken place over the years, to our knowledge, the effectiveness of these antibodies in prevention of the biofilm formed by different S. mutans isolates of the same serotype has not been investigated. Nine clinical isolates of S. mutans were obtained, all serotype c. Gtf-B was purified and together with one of the clinical isolate (SM4), were used as antigen for hen immunization. IgY polyclonal antibodies were purified, and  applied to wells containing a distinct S. mutans isolate. Bacteria growth and biofilm formation was investigated by measuring absorbance at 600 nm and performing a biofilm assay based on crystal violet. Polyclonal antibodies to Gtf-B reduced bacterial growth between 9 and 35% compared to controls in four clinical isolates (P<0.05). As for biofilm formation, the polyclonal antibodies to Gtf-B inhibited biofilms by 17-54%, which was significant for all clinical isolates (p<0.05). On the other hand, polyclonal antibodies to whole-cell S. mutans reduced bacterial growth from 43 to 61%, which was meaningful for all isolates as well as the control strain (SM25175). As for biofilm formation, the anti-whole-cell antibodies reduced biofilm formation by 6-51%, which was significant for only four strains.}, number={2}, journal={International Journal of Industrial Chemistry}, publisher={OICC Press}, author={Varaei, Samaneh Sadat Rasi and Rahimian, Aliasghar and Askari, Hooman and Bahador, Abbas and Pourhajibagher, Maryam and Aminian, Mahdi}, year={2024}, month={Jul.}, keywords={IgY polyclonal antibodies, Dental caries, Streptococcus mutans} }