OICC Press International Nano Letters 2228-5326 9 4 (December 2019) 2019 11 01 Topographic characterization of canine teeth using atomic force microscopy images in nano-scale 10.1007/s40089-019-00284-8 EN Negin Beryani Nezafat Department of Physics, Faculty of Sciences, Science and Research Branch, Islamic Azad University, Tehran, IR Mahmood Ghoranneviss Department of Physics, Faculty of Sciences, Science and Research Branch, Islamic Azad University, Tehran, IR Seyed Mohammad Elahi Department of Physics, Faculty of Sciences, Science and Research Branch, Islamic Azad University, Tehran, IR Azizollah Shafiekhani Physics Department, Alzahra University, Tehran, 1993891167, IR School of Physics, Institute for Research in Fundamental Sciences (IPM), Tehran, IR Zohreh Ghorannevis Department of Physics, Karaj Branch, Islamic Azad University, Karaj, IR Shahram Solaymani Department of Physics, Faculty of Sciences, Science and Research Branch, Islamic Azad University, Tehran, IR School of Physics, Institute for Research in Fundamental Sciences (IPM), Tehran, IR Journal Article 2019 11 01 Abstract The purpose of the present study was to investigate a new method to evaluate micro topography and micro morphology of hard tissue of canine teeth using an atomic force microscope (AFM). For this aim, three extracted human canine teeth were applied. The unpolished surfaces were analyzed with AFM images with 15 µm × 5 µm area and their information obtained by power spectral density (PSD) method and fast Fourier transform algorithm. It was observed that PSD analyses extract suitable information about surface morphological variations so that by moving from enamel to cementum, the fractal dimension and surface complexity were increased.