OICC Press International Nano Letters 2228-5326 5 2 (June 2015) 2015 02 21 Synthesis of Fe3O4 nanoparticles and its antibacterial application 10.1007/s40089-015-0141-z EN Y. T. Prabhu Centre for Nano Science and Technology, Institute of Science and Technology, Jawaharlal Nehru Technological University Hyderabad, Hyderabad, IN K. Venkateswara Rao Centre for Nano Science and Technology, Institute of Science and Technology, Jawaharlal Nehru Technological University Hyderabad, Hyderabad, IN B. Siva Kumari Department of Botany, Andhra Loyola College, Vijayawada, Andhra Pradesh, IN Vemula Sesha Sai Kumar Centre for Nano Science and Technology, Institute of Science and Technology, Jawaharlal Nehru Technological University Hyderabad, Hyderabad, IN Tambur Pavani Centre for Nano Science and Technology, Institute of Science and Technology, Jawaharlal Nehru Technological University Hyderabad, Hyderabad, IN Journal Article 2015 02 21 Abstract The Present work outlines the antibacterial activity of Fe 3 O 4 nanoparticles synthesized through chemical combustion method where ferric nitrate is used as precursor material and urea as fuel with the assistant of Tween 80, a non-ionic surfactant. The obtained Fe 3 O 4 nanoparticles were characterized by X-ray diffraction, differential thermal analysis/thermo gravimetric analysis (DTA/TGA), particle size analyzer, SEM with EDAX and TEM. Various parameters such as dislocation density, micro strain, analysis of weight loss and surface morphological studies were calculated. The particle size was calculated from XRD and it was found to be 33–40 nm. Using well diffusion method antibacterial activity of Fe 3 O 4 nanoparticles was tested against gram-positive and gram-negative Staphylococus aureus , Xanthomonas, Escherichia coli and Proteus vulgaris . Fe 3 O 4 nanoparticles exhibited strong antibacterial activity against bacterial species.