OICC Press International Nano Letters 2228-5326 11 3 (September 2021) 2021 04 11 Response surface methodology-based optimization of Pancratium parvum Dalzell-mediated synthesis of gold nanoparticles with potential biomedical applications 10.1007/s40089-021-00335-z EN Devashree N. Patil Department of Biotechnology, Shivaji University, Kolhapur, IN Prasanna J. Patil School of Food Science and Chemical Engineering, Beijing Technology and Business University (BTBU), Beijing, 100048, CN Manali R. Rane Department of Biotechnology, Shivaji University, Kolhapur, IN Shrirang R. Yadav Department of Botany, Shivaji University, Kolhapur, IN Vishwas A. Bapat Department of Biotechnology, Shivaji University, Kolhapur, IN Govind D. Vyavahare Department of Biotechnology, Shivaji University, Kolhapur, IN Jyoti P. Jadhav Department of Biotechnology, Shivaji University, Kolhapur, IN Journal Article 2021 04 11 Abstract Green nanotechnology is an ever-evolving field of research and development due to an environmentally friendly approach. The present study highlights the green synthesis using Pancratium parvum bulb extract for the biosynthesis of gold nanoparticles and enlightens its thorough characterization with biomedical applications. Here, the biogenic gold nanoparticles (AuNPs) were optimized for maximum and rapid synthesis and characterized by employing several spectroscopic and imaging techniques. Further, the synthesized particles were used to study anti-oxidant, anti-inflammatory, chorioallantoic membrane assay (CAM), anti-acetylcholinesterase enzyme assay, and binding kinetics study, comprising toxicity study on Vero cell lines. The findings concluded that statistically optimized parameters exhibited optimum synthesis of AuNPs with promising anti-oxidant, anti-inflammatory properties, and potential acetylcholinesterase inhibition as a remedy for Alzheimer’s disease. In addition, surface plasmon resonance (SPR) studies revealed good affinity kinetics of immobilized acetylcholinesterase enzyme on sensor chip with KD value 1.449 × 10 –6  M. Concurrently, the effect of AuNPs was evaluated on Vero cell lines, which exhibited the viability of cells at higher concentration, and CAM assay did not demonstrate angiogenicity. Thus, this said approach is rapid with potential medical applications.