OICC Press International Nano Letters 2228-5326 10 1 (March 2020) 2019 12 31 Multifractal analysis of human canine teeth at nano scale: atomic force microscopy studies 10.1007/s40089-019-00293-7 EN Shahram Solaymani Department of Physics, Faculty of Sciences, Science and Research Branch, Islamic Azad University, Tehran, IR Ştefan Ţălu The Directorate of Research, Development and Innovation Management (DMCDI), Technical University of Cluj-Napoca, Cluj-Napoca, Cluj county, 400020, RO Mahmood Ghoranneviss Department of Physics, Faculty of Sciences, Science and Research Branch, Islamic Azad University, Tehran, IR Seyed Mohammad Elahi Department of Physics, Faculty of Sciences, Science and Research Branch, Islamic Azad University, Tehran, IR Azizollah Shafiekhani School of Physics, Institute for Research in Fundamental Sciences (IPM), Tehran, IR Physics Department, Alzahra University, Tehran, 1993891167, IR Mohammadreza Hantehzadeh Department of Physics, Faculty of Sciences, Science and Research Branch, Islamic Azad University, Tehran, IR Negin Beryani Nezafat Department of Physics, Faculty of Sciences, Science and Research Branch, Islamic Azad University, Tehran, IR Journal Article 2019 12 31 Abstract The aim of the present study is to explore the 3-D micromorphology of human canine teeth materials using multifractal analysis through atomic force microscopy (AFM). The 3-D surfaces of ten extracted canine teeth of a group of 40 year old men were studied (enamel, inter enamel, inter dentin, and cementum) by AFM images in tapping mode and on square areas of 1 μm × 1 μm (512 × 512 points). The AFM images and surface multifractal analysis confirm the dependency of surface micromorphology to their structure–property of these materials across the length scales of the teeth structural architecture. Surface statistical parameters and hence, multifractal approach have been considered as reliable and sensitive tools for quantifying the 3-D surface microtexture changes of human canine teeth materials. The surface of inter dentin had the most irregular topography (the width spectrum Δ α  = 2.8361, value bigger than all the other Δ α sample values), while the most regular topography (the width spectrum Δ α  = 2.6804, value lower than all the other sample values) was found in cementum. It has been concluded that multifractal analyses can be used as mathematical tools to explore the 3-D micromorphology of human canine teeth materials.