OICC Press International Nano Letters 2228-5326 3 1 (December 2013) 2013 04 26 Evaluation of synthesized platinum nanoparticles on the MCF-7 and HepG-2 cancer cell lines 10.1186/2228-5326-3-28 EN Hadi Mohammadi Department of Chemistry, Islamic Azad University, North Tehran Branch, Tehran, IR Anita Abedi Department of Chemistry, Islamic Azad University, North Tehran Branch, Tehran, IR Azim Akbarzadeh Department of Pilot Nanobiotechnology, Pasteur Institute of Iran, Tehran, IR Mohammad Javad Mokhtari Department of Biology, Islamic Azad University, Zarghan Branch, Zarghan, IR Hasan Ebrahimi Shahmabadi Department of Pilot Nanobiotechnology, Pasteur Institute of Iran, Tehran, IR Mohamad Reza Mehrabi Department of Pilot Nanobiotechnology, Pasteur Institute of Iran, Tehran, IR Saifuddin Javadian Department of Biochemistry, Pasteur Institute of Iran, Tehran, IR Mohsen Chiani Department of Pilot Nanobiotechnology, Pasteur Institute of Iran, Tehran, IR Journal Article 2013 04 26 Abstract Platinum nanoparticles (PNPs) were synthesized by chemical reduction of potassium hexachloroplatinate (IV) with trisodium citrate under vigorous stirring and addition of sodium dodecyl sulfate as stabilizer reagent. Reducing agent was chosen depending on the oxidation reactions and potential values of the chemical materials used in the experiment. The aim of this study is to investigate the effects of PNPs on the different cancer cell lines and cytotoxicity study of this nanomaterial. The morphology of PNPs was investigated by scanning electron microscope (XL30, Philips Electronics, Amsterdam, The Netherlands) with the ability to perform elemental analysis by EDX. Malvern Zetasizer 3000 HSA (Malvern Instruments, Worcestershire, UK) was used to determine the distribution of particle size and zeta potential of PNPs. The cytotoxicity property of the nanoparticles was evaluated by MTT assay on MCF-7 and HepG-2 cell lines, and the cytotoxic concentration 50% values were determined for 24 h.