OICC Press International Nano Letters 2228-5326 14 2 2024 06 30 Nanotechnology-Enhanced Polymer Scaffolds with Antibacterial and Angiogenic Properties on Diabetic Wound Healing 10.57647/inl.2024.1402.07 EN Hayder Sudan Abbas Obaid Protein Research Center, Shahid Beheshti University, Tehran, Iran; Department of Medical, Hammurabi College of Medicine, University of Babylon, Hillah, Iraq Fateme Mirzajani Protein Research Center, Shahid Beheshti University, Tehran, Iran; Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran Amin Hamidi Protein Research Center, Shahid Beheshti University, Tehran, Iran Iliya Fattahiyan Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran Meysam Omidi Protein Research Center, Shahid Beheshti University, Tehran, Iran Journal Article 2024 06 30 Healing of wounds is a complicated and coordinated process with four phases, namely control of bleeding,inflammation, growth, and regeneration. Chemokines, growth inhibitors, and growth factors are all vital inestablishing cell-cell contacts and the extracellular matrix. The conventional therapies include herbal treatments, specialist dressings,  and interim scaffolds. Polymer scaffolds are a new innovation in wound treatment, providing a three-dimensional, temporary  scaffolding for biological activities during healing. Objectives: A study attempted to speed wound healing processes by combining  angiogenesis-stimulating protein (VEGF), vitamin D, silver carbon quantum dot (Ag-CQD), and henna extract (Lawsonia inermis) on polymeric nanostructures. Characteristics of the manufactured nanostructure investigated using scanning electron microscopy (SEM),  Fourier-transform infrared attenuated total reflectance spectroscopy (FT-IR ATR), and X-ray Powder Diffraction (XRD). The release of Lawson and VEGF (Vascular endothelial growth factor) was measured using high-performance liquid chromatography (HPLC) and  nanodrop micro-volume spectrophotometers. The nanostructures were further strengthened by immobilizing VEGF to increase  angiogenesis at the wound site. Vitamin D and lawsone exhibited more than 70% favorable benefits on wound healing phases. Also  silver nanoparticles demonstrate significant antibacterial activity against gram-positive and gram-negative bacteria. In terms of antibacterial efficacy, this scaffold was evaluated against Staphylococcus aureus and Escherichia coli, and the scaffold Gel/Chi/Pcl/AgCQD/Law/VEGF with 175−200 nm fibers and 10 mg of Lawson was shown to be stable at pH =7.4. Thus, the produced scaffold exhibited considerable resistance against S. aureus, and chitosan mixed with Lawson material appeared to be extremely promising in wound healing.