OICC Press International Nano Letters 2228-5326 4 3 (September 2014) 2014 09 17 Histopathology effects of nickel nanoparticles on lungs, liver, and spleen tissues in male mice 10.1007/s40089-014-0113-8 EN Marziyeh Ajdari Young Researchers and Elite Club, Khorasgan Branch, Islamic Azad University, Isfahan, IR Marziyeh Ziaee Ghahnavieh Department of Biology, Payame Noor University, Tehran, IR Journal Article 2014 09 17 Abstract Because of the classification of the nickel compounds as carcinogenic substances, there is a need for in vivo tests to nickel nanoparticles (NiNPs) for observing their effects on health experimentally. Spherical NiNPs with 10 nm in diameter and 75 ppm concentration were applied for investigating their toxicities within male albino mice as an in vivo model. We randomly made sham group, control group, and 75 ppm group (with five animals in each group). Then, the nanoparticles were injected into mice intraperitonealy for 7 days and after that their lungs, liver, and spleen were removed for histopathological observations. At the end of the test, section microscopic observations of liver, spleen, and lung in sham and control groups showed normal tissues but these tissues underwent significant abnormal effects in 75 ppm group. NiNPs can cause undesirable effects in lungs, liver, and spleen tissues with same condition of this study.