OICC Press International Journal of Nano Dimension 2228-5059 16 1 2025 01 10 Green synthesis, characterization, and biological activities of Copper nanoparticles using Clitoria ternatea leaf extract 1 12 10.57647/j.ijnd.2025.1601.05 EN Rehana Shaik Department of Virology, Sri Venkateswara University, Tirupati-517502, A.P, India https://orcid.org/0009-0006-2499-964X Anitha Buggana Department of Virology, Sri Venkateswara University, Tirupati-517502, A.P, India https://orcid.org/0009-0004-6226-6637 Vijayalakshmi Thalari Department of Virology, Sri Venkateswara University, Tirupati-517502, A.P, India https://orcid.org/0009-0005-3650-0263 Sandhya Rano Department of Virology, Sri Venkateswara University, Tirupati-517502, A.P, India https://orcid.org/0009-0005-6875-2510 Bandari Kedharnath Department of Virology, Sri Venkateswara University, Tirupati-517502, A.P, India https://orcid.org/0009-0008-6532-2701 Narasimha Golla Department of Virology, Sri Venkateswara University, Tirupati-517502, A.P, India https://orcid.org/0000-0002-1760-3321 Journal Article 2025 01 10 Nanotechnology has a broad spectrum in various fields such as chemistry, biology, physics, material sciences, and engineering. Due to the unique features of nanoparticles, it has a wide range of biological applications like Nanomedicine, biosensors, agriculture, and industry. The current study uses an environmentally friendly method of green synthesis of copper nanoparticles using Clitoria ternatea leaf extract. By using sophisticated instruments like UV-visible spectroscopy, FTIR, SEM, and zeta potential, the copper nanoparticles were characterized.  The FTIR spectrum analyzed the functional groups in leaf broth. Scanning Electron Microscope (SEM) confirmed the size and morphology (spherical) of copper nanoparticles. The charge and size of CuNPs were confirmed by Zeta potential. The copper nanoparticles exhibited good antibacterial activity on Gram-positive (Bacillus subtilis) and Gram-negative (E.Coli) bacteria and antifungal properties on fungal strain A. niger. The free radical scavenging activities of copper nanoparticles were studied with DPPH and Hydrogen Peroxide in vitro assay methods. The biogenic CuNPs act as good antioxidants and larvicidal agents.   The antiviral activity of CuNPs was investigated on Sesbania mosaic virus (SeMV), which infects Sesbania plants.  The green synthesized CuNPs act as good antiviral agents for decreasing the mosaic symptoms caused by the Sesbania Mosaic Virus in the Sesbania plant, and it indicates the antiviral efficacy of copper nanoparticles in the Agricultural sector.