OICC Press International Journal of Nano Dimension 2228-5059 15 4 (October 2024) 2024 10 10 Microfluidic Approach for the Synthesis of Silver Nanoparticles as Promising Antimicrobial Agent 10.57647/j.ijnd.2024.1504.32 EN Raffaele Conte National Biodiversity Future Center (NBFC), 90133 Palermo, Italy 0000-0002-6266-4991 Anna Valentino National Biodiversity Future Center (NBFC), 90133 Palermo, Italy 0000-0001-8041-2221 Silvia Romano Elleva Pharma S.R.L. Via Pietro Castellino, 111, 80131 Naples, Italy 0009-0002-2865-6100 Sorur Yazdanpanah Department of Experimental Medicine, Biotechnology and Molecular Biology Section, University of Campania “ Luigi Vanvitelli”, Naples, Italy 0009-0004-7247-0682 Fatima Ez-Zahra Amrati Laboratory of Cell Biology and Molecular Genetics (LBCGM), Department of Biology, Faculty of Sciences,Ibn Zohr University, Agadir 8106, Morocco 0000-0002-3569-0203 Fahd Kandsi Université Mohammed Premier, BV Mohammed VI B.P. 524 Oujda, Maroc 0000-0003-2696-1734 Anna Calarco UniCamillus-Saint Camillus International University of Health Sciences, Rome, Italy 0000-0002-1441-7662 Journal Article 2024 10 10 Silver nanoparticles (AgNPs) have emerged as promising agents in biomedical research due to their unique physicochemical properties and versatile applications. This study focuses on the synthesis of AgNPs using a microfluidic platform, optimizing reaction parameters to achieve monodisperse nanoparticles with a hydrodynamic diameter of approximately 129 nm and a stable negative zeta potential. Characterization techniques including dynamic light scattering and nanoparticles tracking analysis confirmed the size distribution and stability of the synthesized AgNPs. Evaluations of antibacterial activity demonstrated effective inhibition of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa over 72 hours. Biocompatibility assessments on human keratinocytes revealed no cytotoxic effects, supporting the potential of AgNPs for safe biomedical applications. This comprehensive study underscores the significance of AgNPs in nanomedicine, highlighting their role in combating bacterial infections and their promising prospects in therapeutic applications.