OICC Press International Journal of Nano Dimension 2228-5059 9 1 2024 02 28 Pre-concentration of Penicillamine using Fe3O4NPS and analysis of it in drug formulations through spectroscopic method EN Atefeh Mehrabifar Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. Ahmad Riahi Department of Biology, Faculty of Science, Payam Nour University, Tehran, Iran. Journal Article 2024 02 28 First, an effective pre-concentration of penicillamine was done via Fe3O4NPS. Then, a simple, rapid and sensitive spectrophotometric method is described for the determination of penicillamine by 2, 6-dichlorophenolindophenol (2, 6-DCPIP), as the chromogenic agent in bulk drug and formulations. It produces a bluish green coloured compound with maximum absorbance 610 nm. Beer's law was obeyed in the concentration range 0.005-0.100 ppm with molar absorptivity 1.97 × 103 L/mol/cm and RSD 0.3-0.82%. Statistical comparison of the results with those from the reference method reveals excellent agreement and accuracy and precision was ±0.481 to ±0.33%. This method is also applied on the whole blood and serum samples collected from the confirmed patients. The reaction of penicillamine with 2, 6-DCPIP was confirmed by FTIR and 1H NMR. The main advantage of 2, 6-DCPIP was its stability as a reagent solution, resulting in reliable and reproducible results.