OICC Press International Journal of Nano Dimension 2228-5059 9 1 2024 02 28 Hydrothermal synthesis of Copper nanoparticles, characterization and their biological applications EN Kondaiah Seku Department of Chemistry, Malla Reddy Engineering College, Maisammaguda, Secunderbad, Telangana State 500100, India. Bhagavanth Reddy Ganapuram Department of Chemistry, PG Centre Wanaparthy, Palamuru University, Mahabub Nagar, TS, India. Babu Pejjai Department of Information Materials Laboratory (IMSL), School of Chemical Engineering, Yeuungnam University, Gyeongsam 38541, Republic of Korea. Girija Mangatayaru Kotu Department of Chemistry, PG Centre Wanaparthy, Palamuru University, Mahabub Nagar, TS, India. Narasimha Golla Department of Virology, Sri Venkateswra University, Tirupathi, A.P, India. Journal Article 2024 02 28 We report an ecofriendly novel method for copper nanoparticles (CuNPs) synthesis by hydrothermal processes using an activated carboxymethyl gum of locally available medicinal plant Cochlospermum gossypiumas capping agent. The synthesized CuNPs characterized by Ultraviolet - visible Spectroscopy (UV-Vis), Fourier – transform infrared Spectroscopy (FTIR), and Transmission Electron Microscopy (TEM) and X-ray diffraction techniques (XRD). The XRD results showed a face-centered cubic structure with (111) as the prepared orientation. The CuNPs showed good antibacterial and antifungal activity against pathogenic strains like E. coli, Bacillus cereus, Bacillus subtilis and Candida albicans, Candida parapsilosi, Aspergillus Niger and Aspergillus oryzae respectively. A result of this study indicates that the CuNPs has remarkable potential antimicrobial property. It will be used in treating infectious diseases and also use full in biomedical application.