OICC Press International Journal of Nano Dimension 2228-5059 5 6 2024 02 29 Green synthesis of silver nanoparticles using seed aqueous extract of Olea europaea 10.7508/ijnd.2014.06.010 EN B. Sadeghi Department of Chemistry, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran. Journal Article 2024 02 29 Biosynthesis of silver nanoparticles with small size and biostability is very important and used in various biomedical applications. In the present work, we describe the synthesis of silver nanoparticles (Ag-NPs) using seed aqueous extract of Olea europaea (Oe) and its antibacterial activity. UV–visible spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), and X-ray energy dispersive spectrophotometer (EDAX) were performed to ascertain the formation of Ag-NPs. It was observed that the growths of Ag-NPs are stopped within 35 min of reaction time. The synthesized Ag-NPs were characterized by a peak at 449 nm in the UV–visible spectrum. XRD confirmed the crystalline nature of the nanoparticles of 34 nm size. The XRD peaks at 38°, 44°, 64° and 77° can be indexed to the (1 1 1), (2 0 0), (2 2 0) and (3 1 1) Bragg’s reflections of cubic structure of metallic silver, respectively. The results confirmed that the (Oe) is a very good eco friendly and nontoxic source for the synthesis of Ag-NPs as compared to the conventional chemical/physical methods.