OICC Press International Journal of Nano Dimension 2228-5059 5 6 2024 02 29 Synthesis of silver nanoparticles and their antibacterial activity 10.7508/ijnd.2014.06.009 EN A. Zambare Department of Biotechnology, MGM’s Jawaharlal Nehru Engineering College, MGM Campus, N-6, CIDCO, Aurangabad (MS) India. T. Nerpagar Department of Biotechnology, MGM’s Jawaharlal Nehru Engineering College, MGM Campus, N-6, CIDCO, Aurangabad (MS) India. N. Chaudhari Department of Biotechnology, MGM’s Jawaharlal Nehru Engineering College, MGM Campus, N-6, CIDCO, Aurangabad (MS) India. P. Manchalwad Department of Biotechnology, MGM’s Jawaharlal Nehru Engineering College, MGM Campus, N-6, CIDCO, Aurangabad (MS) India. S. Harke Department of Biotechnology, MGM’s Jawaharlal Nehru Engineering College, MGM Campus, N-6, CIDCO, Aurangabad (MS) India. Journal Article 2024 02 29 In study, spherical Silver nanoparticles (SNPs) were synthesized by chemical reduction method from a metal precursor silver nitrate in presence of an anionic surfactant and strong reducing agent. In this experimental work, SNPs are synthesized in presence of different concentration of stabilizing agent and effect of stabilizing agent on size distribution of SNPs have been observed. Further antibacterial activities of the SNPs prepared under different concentration of sodium lauryl sulphate (SDS) carried out on gram negative and gram positive bacteria showed that as concentration of sodium lauryl sulphate increases antibacterial activity decreases.