OICC Press International Journal of Nano Dimension 2228-5059 2 1 2024 03 01 Mushrooms (Agaricus bisporus) mediated biosynthesis of sliver nanoparticles, characterization and their antimicrobial activity 10.7508/ijnd.2011.01.004 EN G. Narasimha Tirupati- 517502, Andhra Pradesh, India B. Praveen Applied Microbiology Laboratory, Department of Virology, Sri Venkateswara University, Tirupati- 517502 Andhra Pradesh, India. K. Mallikarjuna Department of Physics, Sri Venkateswara University, Tirupati-517502 Andhra Pradesh, India. B. Deva Prasad Raju Department of Physics, Sri Venkateswara University, Tirupati-517502 Andhra Pradesh, India. Journal Article 2024 03 01 In this paper we report an eco-friendly route for the synthesis of sliver nanoparticles using Agaricus bisporus (white button mushroom) extract. The synthesized silver nanoparticles were confirmed and characterized by UV-Visible spectrum of the aqueous solution containing silver ions showed a peak at 420 nm corresponding to the surface plasmon absorbance of silver nanoparticles. Transmission Electron Microscopy (TEM) micrographs showed the size of silver nanoparticles and were measured in the range of 8-50nm, Fourier Transform Infrared Spectroscopy (FTIR) spectrum detection of analysis showed peaks between 500-4000 cm-1 confirmed the presence of proteins, carbonyl groups, esters and   carboxylic acids for the synthesis and stabilization of silver nanoparticles .X-ray diffraction (XRD) spectrum of the silver nanoparticles exhibited 2θ values corresponding to the silver nanocrystal. Further, the antibacterial activity of synthesized silver nanoparticles showed effective inhibitory activity against pathogens and non- pathogenic bacterial strains vis, Escherichia coli, Staphylococcus sps, pseudomonas sps, and Bacillus sps.