Microfluidic Approach for the Synthesis of Silver Nanoparticles as Promising Antimicrobial Agent

Abstract

Silver nanoparticles (AgNPs) have emerged as promising agents in biomedical research due to their unique physicochemical properties and versatile applications. This study focuses on the synthesis of AgNPs using a microfluidic platform, optimizing reaction parameters to achieve monodisperse nanoparticles with a hydrodynamic diameter of approximately 129 nm and a stable negative zeta potential. Characterization techniques including dynamic light scattering and nanoparticles tracking analysis confirmed the size distribution and stability of the synthesized AgNPs. Evaluations of antibacterial activity demonstrated effective inhibition of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa over 72 hours. Biocompatibility assessments on human keratinocytes revealed no cytotoxic effects, supporting the potential of AgNPs for safe biomedical applications. This comprehensive study underscores the significance of AgNPs in nanomedicine, highlighting their role in combating bacterial infections and their promising prospects in therapeutic applications.