Microfluidic Approach for the Synthesis of Silver Nanoparticles as Promising Antimicrobial Agent

Abstract

Silver nanoparticles (AgNPs) have emerged as promising agents in biomedical research due to their unique physicochemical properties and versatile applications. This study focuses on the synthesis of AgNPs using a microfluidic platform, optimizing reaction parameters to achieve monodisperse nanoparticles with a hydrodynamic diameter of approximately 129 nm and a stable negative zeta potential. Characterization techniques including dynamic light scattering and nanoparticles tracking analysis confirmed the size distribution and stability of the synthesized AgNPs. Evaluations of antibacterial activity demonstrated effective inhibition of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa over 72 hours. Biocompatibility assessments on human keratinocytes revealed no cytotoxic effects, supporting the potential of AgNPs for safe biomedical applications. This comprehensive study underscores the significance of AgNPs in nanomedicine, highlighting their role in combating bacterial infections and their promising prospects in therapeutic applications.

References

[1]. Hadi S., Mohammad S. S., Mehdi J., Asaad A., Masoud N., Hamed., Amirhossein A. (2020) Genotoxicity assessment of carbon-based nanomaterials; Have their unique physicochemical properties made them double-edged swords. Mutation Research/Reviews in Mutation Research. 783: 108296-108304 ISSN 13835742, doi:10.1016/j.mrrev.2020.108296.

[2]. Naganthran A., Verasoundarapandian G., Khalid F. E., Masarudin M. J., Zulkharnain A., Nawawi N. M., Karim M., Che Abdullah C. A., Ahmad S. A., (2022). Synthesis, Characterization and Biomedical Application of Silver Nanoparticles. Materials.15: 427-432. doi:10.3390/ma15020427

[3]. Shahnaz M., Muthupandian S., Mohammed D., Norul A. Z., Mohamad N. M. I., Ezaz H. R., Sharaf un N. A., Hamed B., Yugal K. M., Ebrahim M. (2023). Bioengineering of green-synthesized TAT peptide-functionalized silver nanoparticles for apoptotic cell-death mediated therapy of breast adenocarcinoma. Talanta. 253: 124026. doi:10.1016/j.talanta.2022.124026.

[4]. Xu L., Wang Y. Y., Huang J., Chen C. Y., Wang Z. X., Xie H., (2020). Silver nanoparticles: Synthesis, medical applications and biosafety. Theranostics.  10: 8996-9031. doi:10.7150/thno.45413

[5]. Shahbazi F., Ahmadi R., Noghani M., Karimi G., (2023). Antibacterial activity of the Iron-Zinc Oxide nanoparticles synthesized via electric discharge method. Int. J. Nano Dimens. 14: 60-72. doi:10.22034/ijnd.2022.1962974.2158

[6]. Paladini F., Pollini M., (2019). Antimicrobial Silver Nanoparticles for Wound Healing Application: Progress and Future Trends. Materials. 12: 2540-2545. doi:10.3390/ma12162540

[7]. Niculescu A. G., Chircov C., Bîrcă A. C., Grumezescu A. M. (2021). Fabrication and Applications of Microfluidic Devices: A Review. Int. J. Mol. Sci. 22: 2011. doi:10.3390/ijms22042011

[8]. Bezelya A., Berrin K., Asuman B., (2023). Microfluidic Devices for Precision Nanoparticle Production. Micro. 3: 822-866. doi:10.3390/micro3040058

[9]. Conte, R., Valentino, A., Di Cristo, F., Peluso, G., Cerruti, P., Di Salle, A., Calarco, A., (2020), Cationic Polymer Nanoparticles-Mediated Delivery of miR-124 Impairs Tumorigenicity of Prostate Cancer Cells. Int. J. Molec. Sci. 21: 153-158. doi:10.3390/ijms21030869

[10]. Di Salle, A.; Viscusi, G.; Di Cristo, F.; Valentino, A.; Gorrasi, G.; Lamberti, E.; Vittoria, V.; Calarco, A.; Peluso, G., (2021). Antimicrobial and Antibiofilm Activity of Curcumin-Loaded Electrospun Nanofibers for the Prevention of the Biofilm-Associated Infections. Molecules, 26: 4866-4871. doi:10.3390/molecules26164866.

[11]. Hamed B., Kamyar J., Elaheh P., Hamed M., Negar B., Hossein V. (2023). Chapter 17 - Bioengineered metal-based antimicrobial nanomaterials for surface coatings,Editor(s): Aditya Kumar, Ajit Behera, Tuan Anh Nguyen, Muhammad Bilal, Ram K. Gupta, Antiviral and Antimicrobial Smart Coatings, Elsevier, Pages 489-539, doi:10.1016/B978-0-323-99291-6.00012-8.

[12] Vernet-Crua A., Medina Cruz D., Mostafavi E., Truong L. B., Barabadi H., Cholula-Díaz J. L., Guisbiers G., Webster T. J., (2023) Chapter 11 - Green-synthesized metallic nanoparticles for antimicrobial applications, Editor(s): Thomas J. Webster, In Woodhead Publishing Series in Biomaterials, Nanomedicine (Second Edition), Woodhead Publishing, Pages 297-338, doi:10.1016/B978-0-12-818627-5.00014-2.

[13] Truong L. B, Medina-Cruz D., Martínez-Sanmiguel J. J., Soto-Mendoza A., Esquivel-López I. G., Pérez Y., Saravanan M., Barabadi H., Cholula-Díaz J. L., Mostafavi E. (2022). Chapter 8 - Biogenic metal nanomaterials to combat antimicrobial resistance, Editor(s): Muthupandian Saravanan, Hamed Barabadi, Ebrahim Mostafavi, Thomas Webster, In Micro and Nano Technologies, Emerging Nanomaterials and Nano-Based Drug Delivery Approaches to Combat Antimicrobial Resistance, Elsevier, Pages 261-304, doi:10.1016/B978-0-323-90792-7.00011-7.

[14]. Dove Autumn S. , Dzurny Dominika I. , Dees Wren R. , Qin Nan , Nunez Rodriguez Carmen C. , Alt Lauren A. , Ellward Garrett L. , Best Jacob A. , Rudawski Nicholas G. , Fujii Kotaro , Czyż Daniel M. (2023). Silver nanoparticles enhance the efficacy of aminoglycosides against antibiotic-resistant bacteria. Frontiers in Microbiology. 13: 1064095. doi:10.3389/fmicb.2022.1064095

[15]. Nqakala, Zimkhitha B., Nicole R. S. Sibuyi, Adewale O. Fadaka, Mervin Meyer, Martin O. Onani, and Abram M. Madiehe. (2021). Advances in Nanotechnology towards Development of Silver Nanoparticle-Based Wound-Healing Agents. Int. J. Molec. Sci. 22: 11272. doi:10.3390/ijms222011272

[16]. Ong W. T. J., Nyam K. L. (2022). Evaluation of silver nanoparticles in cosmeceutical and potential biosafety complications. Saudi. J. Biol. Sci. 29: 2085-2094. doi:10.1016/j.sjbs.2022.01.035

[17]. Asif M., Yasmin R., Asif R., Ambreen A., Mustafa M., Umbreen S., (2022). Green Synthesis of Silver Nanoparticles (AgNPs), Structural Characterization, and their Antibacterial Potential. Dose Response. 20: 143-156. doi:10.1177/15593258221088709

[18]. Bîrcă A. C., Gherasim O., Niculescu A. G., Grumezescu A. M., Neacu I. A., Chircov C., Vasile B., Oprea O. C., Andronescu E., Stan M. S., Curuiu C., Diu L. M., Holban A. M., (2023), A Microfluidic Approach for Synthesis of Silver Nanoparticles as a Potential Antimicrobial Agent in Alginate-Hyaluronic Acid-Based Wound Dressings. Int. J. Mol. Sci. 24: 11466-11472. doi:10.3390/ijms241411466

[19]. Conte, R., De Luca I., Valentino A., Cerruti P., Pedram P., Cabrera-Barjas G., Moeini A., and Calarco A. (2023). "Hyaluronic Acid Hydrogel Containing Resveratrol-Loaded Chitosan Nanoparticles as an Adjuvant in Atopic Dermatitis Treatment". J Func. Biomater. 14: 82-87. doi:10.3390/jfb14020082

[20].Alimoradi, M., Yousefi, M., Sadeghi, B., Amini M. M., Abbasi, A. (2019). Structural and Magnetic Behavior of BaAlxCryFe11O19 (x+y=1) Hexagonal Ferrites. J.  Supercond Nov, Magn. 32: 2533–2538 doi:10.1007/s10948-018-4980-5

[21]. Helmlinger,J., Sengstock, C., Groß-Heitfeld, C., Mayer, C:, Schildhauer, T. A. , Köllerb M., Epple M.(2016).  Silver nanoparticles with different size and shape: equal cytotoxicity, but different antibacterial effects. RSC Adv. 6: 18490-18501. doi:10.1039/C5RA27836H

[22]. Piatek, Magdalena, Cillian O’Beirne, Zoe Beato, Matthias Tacke, and Kevin Kavanagh. (2023). Pseudomonas aeruginosa and Staphylococcus aureus Display Differential Proteomic Responses to the Silver (I) Compound, SBC3. Antibiotics. 12: 348-355. doi:10.3390/antibiotics12020348

[23]. Yang, E., Fan, L., Yan, J., Jiang Y., Doucette C., Filmore S., Walker B. (2018).Influence of culture media, pH and temperature on growth and bacteriocin production of bacteriocinogenic lactic acid bacteria. AMB Expr. 8: 10-14. doi:10.1186/s13568-018-0536-0

[24]. Zielińska-Górska M., Sawosz E., Sosnowska M., Hotowy A., Grodzik M., Górski K., Strojny-Cieślak B., Wierzbicki M., Chwalibog A.(2022)  Molecular Biocompatibility of a Silver Nanoparticle Complex with Graphene Oxide to Human Skin in a 3D Epidermis In Vitro Model. Pharmaceutics. 14: 1398-1403. doi: 10.3390/pharmaceutics14071398.