OICC Press International Journal of Energy and Environmental Engineering 2251-6832 5 1 (April 2014) 2014 01 24 Impacts of pH, temperature, and pretreatment method on biohydrogen production from organic wastes by sewage microflora 10.1186/2251-6832-5-6 EN Jaruwan Wongthanate Faculty of Environment and Resource Studies, Mahidol University, Salaya, Phutthamonthon, Nakhon Pathom, 73170, TH Kittibodee Chinnacotpong Faculty of Environment and Resource Studies, Mahidol University, Salaya, Phutthamonthon, Nakhon Pathom, 73170, TH Madsamon Khumpong Faculty of Environment and Resource Studies, Mahidol University, Salaya, Phutthamonthon, Nakhon Pathom, 73170, TH Journal Article 2014 01 24 Abstract Biohydrogen production could be generated from organic wastes - food and beverage processing wastewater, restaurant food waste, and raw starch waste. Fermentative hydrogen production from food and beverage processing wastewater by sewage microflora was optimized in terms of pH (4.5 to 7.0), mesophilic condition (35°C ±2°C), and thermophilic condition (50°C ±2°C). Low initial pH (6.5) and mesophilic condition favored hydrogen production (0.28 L/L) indicating that such parameters along with the wastewater characteristics were crucial to dark-fermentative hydrogen production. Pretreatment methods (methanogenic inhibitor, sterilization, sonication, and acidification) on restaurant food waste and raw starch waste to enhance biohydrogen production were also investigated in this study. Maximum hydrogen yields of 3.48 ml H 2 /g COD and 2.18 ml H 2 /g COD were observed in sterilization of pretreated restaurant food and raw starch wastes, respectively.