OICC Press International Journal of Energy and Environmental Engineering 2251-6832 3 1 (December 2012) 2012 08 31 Dilute H2SO4-catalyzed hydrothermal pretreatment to enhance enzymatic digestibility of Jatropha curcas fruit hull for ethanol fermentation 10.1186/2251-6832-3-15 EN Ahmad Marasabessy Agrotechnology and Food Sciences Group, Biobased Commodity Chemicals, Wageningen University, Wageningen, 6700 AA, NL Department of Chemical Engineering, Rijksuniversiteit Groningen, Groningen, 9747 AG, NL Agency for the Assessment and Application of Technology (BPPT), Jakarta, 10340, ID A Maarten J Kootstra Agrotechnology and Food Sciences Group, Biobased Commodity Chemicals, Wageningen University, Wageningen, 6700 AA, NL Bioprocess Engineering Group, Wageningen University, Wageningen, 6700 AA, NL Johan PM Sanders Agrotechnology and Food Sciences Group, Biobased Commodity Chemicals, Wageningen University, Wageningen, 6700 AA, NL Ruud A Weusthuis Agrotechnology and Food Sciences Group, Biobased Commodity Chemicals, Wageningen University, Wageningen, 6700 AA, NL Journal Article 2012 08 31 Abstract Dilute sulfuric acid pretreatment of the Jatropha curcas fruit hull at high temperatures (140°C to 180°C) performed in a 110-mL stainless steel reactor was investigated to enhance the enzymatic digestibility of its lignocellulosic components. Carbohydrates accounted for 43% of the dry matter of the J. curcas fruit hull biomass. The goal of the study was to optimize the pretreatment conditions (acid concentration, time, and temperature) in order to obtain the highest sugar yield after subsequent enzymatic hydrolysis. A Box-Behnken design was applied to the experimental setup in order to reduce the number of experiments. The optimal pretreatment conditions are 30-min incubations at a temperature of 178°C with a sulfuric acid concentration of 0.9% ( w / v ). Using these pretreatment conditions for a fruit solid loading of 9.52% followed by a 24-h enzymatic hydrolysis resulted in a liberation of 100% of all pentoses present (71% yield and 29% degradation to furfural) and 83% of the hexoses (78% yield and 5% degradation to 5-hydroxymethylfurfural). The simultaneous saccharification and fermentation experiment showed that acid-pretreated fruit hull can be used as a substrate for Saccharomyces cerevisiae to produce ethanol.