OICC Press Iranian Journal of Catalysis 2345-4865 15 1 2025 02 08 Facile synthesis of silver/iron-TiO2 nanoparticles for sterilization of Nodal explants of Dracaena sanderiana Sander ex Mast. cvs ‘Gold’ and ‘Victory’ and growth regulator effects on in vitro growth 10.57647/j.ijc.2025.1501.11 EN Kasun L. Seneviratne Integrated Watershed and Water Resources Management Project (IWWRMP), Ministry of Irrigation, Colombo, Sri Lanka https://orcid.org/0000-0002-0038-3289 Sriyani E. Peiris Faculty of Humanities and Sciences, Sri Lanka Institute of Information Technology (SLIIT), New Kandy Road, Malabe, Sri Lanka https://orcid.org/0000-0001-8691-680X Colin N. Peiris Faculty of Humanities and Sciences, Sri Lanka Institute of Information Technology (SLIIT), New Kandy Road, Malabe, Sri Lanka https://orcid.org/0000-0001-7807-6062 R.P.A. Shashikala Faculty of Humanities and Sciences, Sri Lanka Institute of Information Technology (SLIIT), New Kandy Road, Malabe, Sri Lanka Sehan Jayasinghe Faculty of Humanities and Sciences, Sri Lanka Institute of Information Technology (SLIIT), New Kandy Road, Malabe, Sri Lanka https://orcid.org/0009-0005-1769-8710 Nilwala Kottegoda Department of Chemistry, Faculty of Applied Sciences, University of Sri Jayewardenepura, Colombo, Sri Lanka https://orcid.org/0000-0002-9664-1704 Journal Article 2025 02 08 This study investigates the application of silver/iron-titanate (Ag/Fe-TiO2) nanoparticles (NPs) as a sterilizing agent for in vitro cultures of Dracaena sanderiana cultivars ‘Gold’ and ‘Victory’. The motivation behind this research is to find an effective sterilization method that does not harm plant growth. The Ag/Fe-TiO2 NPs, activated by visible light, exhibit catalytic properties that eliminate harmful microbes such as Escherichia coli, Staphylococcus aureus, and Fusarium spp. The main findings indicate that the combination of Benzyl Amino Purine (BAP) at 1.5 mg L-1 and Indole Acetic Acid (IAA) at 0.01 mg L-1 significantly enhances shoot number and length in both cultivars. The use of 200 mg L-1 Ag/Fe-TiO2 NPs achieved 90% contamination-free cultures in the first cycle, with a slight decrease to 80% in the second cycle and 70% in the third cycle. Importantly, the treatment not only sterilizes but also promotes plant growth, suggesting that Ag/Fe-TiO2 NPs could be a sustainable solution for in vitro plant mass production. Research Highlights Visible-light-driven Ag/Fe-TiO2 NPs show significant antibacterial and antifungal activity. Antimicrobial action of Ag/Fe-TiO2 NPs is utilized for in vitro micro-propagation. The proposed Ag/Fe-TiO2 NPs treatment eliminates the bacteria and fungus sp. adhere to the plant node surface. The growth regulator combination (BAP + IAA) enhanced the shoot multiplication and shoot growth with both species. Enhance the micropropergation method.