10.57647/tpr.2025.0903.15

Unveiling the potential antidiabetic, antidyslipidemic, and antimicrobial effects of methanolic root extracts of Nymphaea pubescens Willd. plant: Evidence from in-vitro and in-vivo approaches

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  • Sahid Ahmad Santo1,
  • Tanmay Deb1,
  • Md. Abdus Salam1,
  • Arafat Miah1,
  • Md. Saddam Hussain1,
  • Anamika Datta2,
  • Mohammad Nurul Amin3,
  • Mohammad Sarowar Uddin1,
  • Mohammad Safiqul Islam1,
  • Md. Shalahuddin Millat*1,
  1. Department of Pharmacy, Noakhali Science and Technology University, Noakhali, Bangladesh
  2. Department of Pharmacy, R. P. Shaha University, Narayanganj, Bangladesh
  3. Department of Pharmacy, Atish Dipankar University of Science and Technology, Dhaka, Bangladesh

Received: 2024-12-27

Revised: 2025-09-26

Accepted: 2025-09-27

Published in Issue 2025-09-30

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

Santo, S. A., Deb, T., Salam, M. A., Miah, A., Hussain, M. S., Datta, A., Amin, M. N., Uddin, M. S., Islam, M. S., & Millat, M. S. (2025). Unveiling the potential antidiabetic, antidyslipidemic, and antimicrobial effects of methanolic root extracts of Nymphaea pubescens Willd. plant: Evidence from in-vitro and in-vivo approaches. Trends in Phytochemical Research, 9(3), 181-190. https://doi.org/10.57647/tpr.2025.0903.15
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Abstract

This study investigated the therapeutic potential of methanolic root extracts of Nymphaea pubescens Willd. with a focus on their antidiabetic, antilipidemic, and antimicrobial properties. The methanolic root extracts of the N. pubescens at higher doses (500 mg/kg) exhibited a pronounced serum blood glucose-lowering activity (p < 0.001) and also demonstrated remarkable outcomes in body weight loss (p < 0.01), closely resembling the effects of the standard metformin. The same experiment displayed a noticeable reduction in low-density lipoprotein cholesterol (LDL-C) and total cholesterol (TC) levels (p < 0.001), along with a notable elevation in high-density lipoprotein cholesterol (HDL-C) levels (p < 0.01). Furthermore, the plant extract at 500 mg/kg unveiled significant antimicrobial activities (p < 0.001) against both Staphylococcus aureus and Aspergillus niger and also exhibited significant (p < 0.01) inhibition of Escherichia coli. In summary, these findings highlight the multifaceted therapeutic potential of N. pubescens root extracts, warranting further investigation for their possible development into novel therapeutic agents.

Keywords

  • Antidiabetic; Antihyperlipidemic; Antimicrobial; In vitro; In vivo; Nymphaea pubescens Willd.; Nymphaeaceae
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