10.1007/s40204-016-0060-8

Fabrication and characterization of biosilver nanoparticles loaded calcium pectinate nano-micro dual-porous antibacterial wound dressings

  1. International and Inter University Centre for Nanoscience and Nanotechnology, Mahatma Gandhi University, Kottayam, Kerala, 686560, IN School of Nano Science and Technology, National Institute of Technology Calicut, Calicut, Kerala, 673601, IN
  2. Department of Chemistry, Bishop Kurialacherry College for Women, Kottayam, Kerala, 686561, IN
  3. International and Inter University Centre for Nanoscience and Nanotechnology, Mahatma Gandhi University, Kottayam, Kerala, 686560, IN School of Pure and Applied Physics, Mahatma Gandhi University, Kottayam, Kerala, 686560, IN
  4. International and Inter University Centre for Nanoscience and Nanotechnology, Mahatma Gandhi University, Kottayam, Kerala, 686560, IN School of Chemical Sciences, Mahatma Gandhi University, Kottayam, Kerala, 686560, IN
Cover Image

Published in Issue 2016-12-02

How to Cite

Augustine, R., Augustine, A., Kalarikkal, N., & Thomas, S. (2016). Fabrication and characterization of biosilver nanoparticles loaded calcium pectinate nano-micro dual-porous antibacterial wound dressings. Progress in Biomaterials, 5(3-4 (December 2016). https://doi.org/10.1007/s40204-016-0060-8

Abstract

Abstract Development of materials for medical applications using biologically derived materials by green approaches is emerging as an important focus in the present healthcare scenario. Herein the first time, we report the plant extract mediated ultra-rapid biosynthesis of silver nanoparticles using whole plant extracts of Biophytum sensitivum . Synthesized nanoparticles were immobilized in nano-micro dual-porous calcium pectinate scaffolds for wound dressing application. Pectinate wound dressings containing silver nanoparticles have shown excellent antibacterial property and exudate uptake capacity while being biocompatible to the human cells.

Keywords

  • Silver nanoparticles,
  • Biophytum,
  • Biosynthesis,
  • Pectinate,
  • Wound dressings

References

  1. AshaRani et al. (2008) Cytotoxicity and genotoxicity of silver nanoparticles in human cells 3(2) (pp. 279-290) https://doi.org/10.1021/nn800596w
  2. Augustine and Rajarathinam (2012) Synthesis and characterization of silver nanoparticles and its immobilization on alginate coated sutures for the prevention of surgical wound infections and the in vitro release studies 2(3) (pp. 205-212)
  3. Augustine R, Rajendran R, Cvelbar U, Mozeticˇ M, George A (2013) Biopolymers for health, food, and cosmetic applications. In: Thomas S, Durand D, Chassenieux C, Jyotishkumar P (eds) Handbook of biopolymer-based materials: from blends and composites to gels and complex networks. Wiley, pp 801–849
  4. Augustine et al. (2014) Electrospun polycaprolactone membranes incorporated with ZnO nanoparticles as skin substitutes with enhanced fibroblast proliferation and wound healing 4(47) (pp. 24777-24785) https://doi.org/10.1039/c4ra02450h
  5. Augustine et al. (2014) Advancement of wound care from grafts to bioengineered smart skin substitutes 3(2–4) (pp. 103-113) https://doi.org/10.1007/s40204-014-0030-y
  6. Augustine et al. (2014) Role of wound dressings in the management of chronic and acute diabetic wounds (pp. 273-314) Apple Academic Press https://doi.org/10.1201/b16415-8
  7. Augustine et al. (2014) A facile and rapid method for the black pepper leaf mediated green synthesis of silver nanoparticles and the antimicrobial study 4(7) (pp. 809-818) https://doi.org/10.1007/s13204-013-0260-7
  8. Augustine et al. (2015) Electrospun poly (ε-caprolactone)-based skin substitutes: in vivo evaluation of wound healing and the mechanism of cell proliferation 103(7) (pp. 1445-1454) https://doi.org/10.1002/jbm.b.33325
  9. Augustine et al. (2015) Polyuronates and their application in drug delivery and cosmetics (pp. 239-269) Apple Academic Press https://doi.org/10.1201/b19772-9
  10. Augustine et al. (2015) Electrospun PCL membranes incorporated with biosynthesized silver nanoparticles as antibacterial wound dressings 6(3) (pp. 337-344) https://doi.org/10.1007/s13204-015-0439-1
  11. Augustine et al. (2015) An in vitro method for the determination of microbial barrier property (MBP) of porous polymeric membranes for skin substitute and wound dressing applications 12(1) (pp. 12-19) https://doi.org/10.1007/s13770-014-0032-9
  12. Awwad et al. (2012) Biosynthesis of silver nanoparticles using Olea europaea leaves extract and its antibacterial activity 2(6) (pp. 164-170) https://doi.org/10.5923/j.nn.20120206.03
  13. Awwad et al. (2013) Green synthesis of silver nanoparticles using carob leaf extract and its antibacterial activity 4(1) (pp. 1-6) https://doi.org/10.1186/2228-5547-4-29
  14. Burd et al. (2007) A comparative study of the cytotoxicity of silver-based dressings in monolayer cell, tissue explant, and animal models 15(1) (pp. 94-104) https://doi.org/10.1111/j.1524-475X.2006.00190.x
  15. Chandran et al. (2006) Synthesis of gold nanotriangles and silver nanoparticles using Aloevera plant extract 22(2) (pp. 577-583) https://doi.org/10.1021/bp0501423
  16. Fan et al. (2013) Mechanically strong graphene oxide/sodium alginate/polyacrylamide nanocomposite hydrogel with improved dye adsorption capacity 1(25) (pp. 7433-7443) https://doi.org/10.1039/c3ta10639j
  17. Fayaz et al. (2009) Fungal based synthesis of silver nanoparticles—an effect of temperature on the size of particles (pp. 123-126) https://doi.org/10.1016/j.colsurfb.2009.07.002
  18. Foldbjerg et al. (2011) Cytotoxicity and genotoxicity of silver nanoparticles in the human lung cancer cell line, A549 85(7) (pp. 743-750) https://doi.org/10.1007/s00204-010-0545-5
  19. Fuchs (2016) Epithelial skin biology: three decades of developmental biology, a hundred questions answered and a thousand new ones to address https://doi.org/10.1016/bs.ctdb.2015.11.033
  20. Guo et al. (2014) Characteristics of two calcium pectinates prepared from citrus pectin using either calcium chloride or calcium hydroxide 62(27) (pp. 6354-6361) https://doi.org/10.1021/jf5004545
  21. Unknown (2007) PA
  22. Jadhav et al. (2016) Green and ecofriendly synthesis of silver nanoparticles: characterization, biocompatibility studies and gel formulation for treatment of infections in burns (pp. 109-115) https://doi.org/10.1016/j.jphotobiol.2016.01.002
  23. Jha et al. (2009) Plant system: nature’s nanofactory 73(2) (pp. 219-223) https://doi.org/10.1016/j.colsurfb.2009.05.018
  24. Khalil et al. (2014) Green synthesis of silver nanoparticles using olive leaf extract and its antibacterial activity 7(6) (pp. 1131-1139) https://doi.org/10.1016/j.arabjc.2013.04.007
  25. Kim et al. (2011) Antibacterial activity of silver-nanoparticles against Staphylococcus aureus and Escherichia coli 39(1) (pp. 77-85)
  26. Latha et al. (2016) Biocompatibility and antibacterial activity of the Adathoda vasica Linn extract mediated silver nanoparticles (pp. 88-94) https://doi.org/10.1016/j.micpath.2016.01.013
  27. Lee et al. (2009) Fatty acid synthase inhibition by amentoflavone induces apoptosis and antiproliferation in human breast cancer cells 32(8) (pp. 1427-1432) https://doi.org/10.1248/bpb.32.1427
  28. Lee et al. (2013) Fatty acid synthase inhibition by amentoflavone suppresses HER2/neu (erbB2) oncogene in SKBR3 human breast cancer cells 27(5) (pp. 713-720) https://doi.org/10.1002/ptr.4778
  29. Li et al. (2007) Reexamining the egg-box model in calcium-alginate gels with X-ray diffraction 8(2) (pp. 464-468) https://doi.org/10.1021/bm060550a
  30. Lloyd et al. (1998) Carbohydrate polymers as wound management aids 37(3) (pp. 315-322) https://doi.org/10.1016/S0144-8617(98)00077-0
  31. Mallik et al. (2001) Seed mediated formation of bimetallic nanoparticles by UV irradiation: a photochemical approach for the preparation of “core-shell” type structures 1(6) (pp. 319-322) https://doi.org/10.1021/nl0100264
  32. May (1990) Industrial pectins: sources, production and applications 12(1) (pp. 79-99) https://doi.org/10.1016/0144-8617(90)90105-2
  33. McFarland (1907) The nephelometer: an instrument for estimating the number of bacteria in suspensions for calculating the opsonic index and vaccines (pp. 1176-1178) https://doi.org/10.1001/jama.1907.25320140022001f
  34. Mi et al. (2001) Fabrication and characterization of a sponge-like asymmetric chitosan membrane as a wound dressing 22(2) (pp. 165-173) https://doi.org/10.1016/S0142-9612(00)00167-8
  35. Miraftab et al. (2003) Fibres for wound dressings based on mixed carbohydrate polymer fibres 53(3) (pp. 225-231) https://doi.org/10.1016/S0144-8617(03)00108-5
  36. Mishra et al. (2011) Development and characterization of pectin/gelatin hydrogel membranes for wound dressing 15(1) (pp. 82-95) https://doi.org/10.1007/s12588-011-9016-y
  37. Mohanpuria et al. (2008) Biosynthesis of nanoparticles: technological concepts and future applications 10(3) (pp. 507-517) https://doi.org/10.1007/s11051-007-9275-x
  38. Mollick MMR, Rana D, Dash SK, Chattopadhyay S, Bhowmick B, Maity D, Chattopadhyay D (2015) Studies on green synthesized silver nanoparticles using
  39. Abelmoschus esculentus
  40. (L.) pulp extract having anticancer (in vitro) and antimicrobial applications. Arab J Chem. doi:
  41. 10.1016/j.arabjc.2015.04.033
  42. MubarakAli et al. (2011) Plant extract mediated synthesis of silver and gold nanoparticles and its antibacterial activity against clinically isolated pathogens 85(2) (pp. 360-365) https://doi.org/10.1016/j.colsurfb.2011.03.009
  43. Natarajan et al. (2010) Antibacterial activity of leaf extracts of Biophytum sensitivum (L.) DC 2(11) (pp. 717-720)
  44. Pana´cˇek et al. (2006) Silver colloid nanoparticles: synthesis, characterization, and their antibacterial activity 110(33) (pp. 16248-16253) https://doi.org/10.1021/jp063826h
  45. Park (2014) New paradigm shift for the green synthesis of antibacterial silver nanoparticles utilizing plant extracts 30(3) https://doi.org/10.5487/TR.2014.30.3.169
  46. Pillai and Kamat (2004) What factors control the size and shape of silver nanoparticles in the citrate ion reduction method? 108(3) (pp. 945-951) https://doi.org/10.1021/jp037018r
  47. Prabhu and Poulose (2012) Silver nanoparticles: mechanism of antimicrobial action, synthesis, medical applications, and toxicity effects 2(1) (pp. 1-10) https://doi.org/10.1186/2228-5326-2-32
  48. Rai et al. (2006) FTIR, Raman spectra and ab initio calculations of 2-mercaptobenzothiazole 63(2) (pp. 483-490) https://doi.org/10.1016/j.saa.2005.05.034
  49. Rai et al. (2009) Silver nanoparticles as a new generation of antimicrobials 27(1) (pp. 76-83) https://doi.org/10.1016/j.biotechadv.2008.09.002
  50. Sakthivel and Guruvayoorappan (2012) Biophytum sensitivum: ancient medicine, modern targets 3(2) https://doi.org/10.4103/2231-4040.97279
  51. Saravanan et al. (2011) Rapid biosynthesis of silver nanoparticles from Bacillus megaterium (NCIM 2326) and their antibacterial activity on multi drug resistant clinical pathogens 88(1) (pp. 325-331) https://doi.org/10.1016/j.colsurfb.2011.07.009
  52. Sathyavathi et al. (2010) Biosynthesis of silver nanoparticles using Coriandrum sativum leaf extract and their application in nonlinear optics 3(2) (pp. 138-143) https://doi.org/10.1166/asl.2010.1099
  53. Sherry et al. (2005) Localized surface plasmon resonance spectroscopy of single silver nanocubes 5(10) (pp. 2034-2038) https://doi.org/10.1021/nl0515753
  54. Shrivastava et al. (2007) Characterization of enhanced antibacterial effects of novel silver nanoparticles 18(22) https://doi.org/10.1088/0957-4484/18/22/225103
  55. Song and Kim (2009) Rapid biological synthesis of silver nanoparticles using plant leaf extracts (pp. 79-84) https://doi.org/10.1007/s00449-008-0224-6
  56. Sundaram et al. (2012) Extracellular biosynthesis of iron oxide nanoparticles by Bacillus subtilis strains isolated from rhizosphere soil 17(4) (pp. 835-840) https://doi.org/10.1007/s12257-011-0582-9
  57. Tummalapalli et al. (2016) Composite wound dressings of pectin and gelatin with aloe vera and curcumin as bioactive agents (pp. 104-113) https://doi.org/10.1016/j.ijbiomac.2015.10.087
  58. Unnithan et al. (2012) Wound-dressing materials with antibacterial activity from electrospun polyurethane–dextran nanofiber mats containing ciprofloxacin HCl 90(4) (pp. 1786-1793) https://doi.org/10.1016/j.carbpol.2012.07.071
  59. Wang et al. (2007) Synthesis and characterization of Ag nanoparticles assembled in ordered array pores of porous anodic alumina by chemical deposition (pp. 3795-3797) https://doi.org/10.1016/j.matlet.2006.12.035
  60. Wilsky et al. (2012) Inhibition of fatty acid synthase by amentoflavone reduces coxsackievirus B3 replication 157(2) (pp. 259-269) https://doi.org/10.1007/s00705-011-1164-z
  61. Xu et al. (2015) Development of tannic acid/chitosan/pullulan composite nanofibers from aqueous solution for potential applications as wound dressing (pp. 16-24) https://doi.org/10.1016/j.carbpol.2014.08.081