10.1007/s40204-016-0059-1

Comparative of fibroblast and osteoblast cells adhesion on surface modified nanofibrous substrates based on polycaprolactone

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  • Fereshteh Sharifi1,
  • Shiva Irani1,
  • Mojgan Zandi*2,
  • Masoud Soleimani3,
  • Seyed Mohammad Atyabi4,
  1. Department of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, IR
  2. Department of Biomaterials, Iran Polymer and Petrochemical Institute, Tehran, IR
  3. Department of Hematology, Faculty of Medical Science, Tarbiat Moddares University, Tehran, IR
  4. Department of Pilot Biotechnology, Pasteur Institute of Iran, Tehran, IR
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Published in Issue 2016-12-08

How to Cite

Sharifi, F., Irani, S., Zandi, M., Soleimani, M., & Atyabi, S. M. (2016). Comparative of fibroblast and osteoblast cells adhesion on surface modified nanofibrous substrates based on polycaprolactone. Progress in Biomaterials, 5(3-4 (December 2016). https://doi.org/10.1007/s40204-016-0059-1
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Abstract

Abstract One of the determinant factors for successful bioengineering is to achieve appropriate nano-topography and three-dimensional substrate. In this research, polycaprolactone (PCL) nano-fibrous mat with different roughness modified with O 2 plasma was fabricated via electrospinning. The purpose of this study was to evaluate the effect of plasma modification along with surface nano-topography of mats on the quality of human fibroblast (HDFs) and osteoblast cells (OSTs)-substrate interaction. Surface properties were studied using scanning electron microscopy (SEM), atomic force microscopy (AFM), contact angle, Fourier-transformation infrared spectroscopy. We evaluated mechanical properties of fabricated mats by tensile test. The viability and proliferation of HDFs and OSTs on the substrates were followed by 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT). Mineralization of the substrate was determined by alizarin red staining method and calcium content of OSTs was determined by calcium content kit. Cells morphology was studied by SEM analysis. The results revealed that the plasma-treated electrospun nano-fibrous substrate with higher roughness was an excellent designed substrate. A bioactive topography for stimulating proliferation of HDFs and OSTs is to accelerate the latter’s differentiation time. Therefore, the PCL substrate with high density and major nano-topography were considered as a bio-functional and elegant bio-substrate for tissue regeneration applications.

Keywords

  • Plasma treatment,
  • Nano-topography,
  • Electrospinning,
  • Human fibroblast cells (HDFs),
  • Osteoblast cells (OSTs)
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