10.1007/s40204-016-0058-2

Roll-designed 3D nanofibrous scaffold suitable for the regeneration of load bearing bone defects

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  • Fatemeh Hejazi1,
  • Hamid Mirzadeh*1,
  1. Department of Polymer Engineering and Color Technology, Amirkabir University of Technology (Tehran Polytechnic), Tehran, 1591634311, IR
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Published in Issue 2016-11-18

How to Cite

Hejazi, F., & Mirzadeh, H. (2016). Roll-designed 3D nanofibrous scaffold suitable for the regeneration of load bearing bone defects. Progress in Biomaterials, 5(3-4 (December 2016). https://doi.org/10.1007/s40204-016-0058-2
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Abstract

Abstract In this work, an innovative and easy method for the fabrication of 3D scaffold from 2D electrospun structures is introduced. For this aim, coral microparticles were fixed inside the nanofibrous PCL/Gelatin mat and the obtained structure was post assembled into a cylindrical design. Scaffold fabrication procedure is described in detail and morphological properties, physical and mechanical characteristics and in vitro assessments of the prepared scaffold are reported. Presences of coral microparticles in the structure led to the formation of empty spaces (3D pores) between nanofibrous layers which in turn prevent the compact accumulation of nanofibers. Post-assembly of the obtained nanofibrous coral-loaded structures makes it possible to prepare a scaffold with any desired dimension (diameter and height). Existence of coral particles within the nanofibrous mats resulted in distant placement of layers toward each other in the assembling step, which in turn create vacancy in the structure for cellular migration and fluid and nutrients exchange of the scaffold with the surrounding environment. Cell morphology within the scaffolds is investigated and cytotoxicity and cytocompatibility of the structure is evaluated using Alamar blue assay. Enhancement in mineralization of the seeded cells within the prepared coral-loaded scaffolds is demonstrated by the use of SEM-EDX. Performed compression mechanical test revealed excellent modulus and stiffness values for the cylindrical samples which are comparable to those of natural bone tissue.

Keywords

  • 3D scaffold,
  • Coral microparticles,
  • Cellular interactions,
  • Mechanical properties
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References

  1. Baker BM, Shah RP, Silverstain AM, Esterhai JL, Burdick JA, Mauck RL (2012) Sacrificial nanofibrous composites provide instruction without impediment and enable functional tissue formation. PNAS 109(35):14176–14181
  2. Beachley et al. (2014) Precisely assembled nanofiber arrays as a platform to engineer aligned cell sheets for biofabrication https://doi.org/10.3390/bioengineering1030114
  3. Binulala et al. (2014) PCL–gelatin composite nanofibers electrospun using diluted acetic acid–ethyl acetate solvent system for stem cell-based bone tissue engineering 25(4) (pp. 325-340) https://doi.org/10.1080/09205063.2013.859872
  4. Cancer Registration & Surveillance Modules (2000) Structure of bone tissue. National Cancer Institute, SEER Training Modules.
  5. http://training.seer.cancer.gov/anatomy/skeletal/tissue.html
  6. . Accessed 1 Oct 2016
  7. Coral Uses, Benefits & Dosage (2005) Drugs.com Herbal Database.
  8. http://www.drugs.com/npp/coral.html
  9. . Accessed 1 Oct 2016
  10. Ch B (2012) The skeletal system: osseous tissue and skeletal structure powerpoint lecture presentations, vol 5. Pearson Education, London
  11. Chomachayi et al. (2016) Electrospun silk-based nanofibrous scaffolds: fiber diameter and oxygen transfer (pp. 71-80) https://doi.org/10.1007/s40204-016-0046-6
  12. Feinstein (1972) Density of a binary mixture. A classroom or laboratory exercise 49(2) https://doi.org/10.1021/ed049p111
  13. Gu et al. (2013) Fabrication of sonicated chitosan nanofiber mat with enlarged porosity for use as hemostatic materials https://doi.org/10.1016/j.carbpol.2013.04.060
  14. Hejazi F, Mirzadeh H (2016) Novel 3D scaffold with enhanced physical and cell response properties for bone tissue regeneration, fabricated by Patterned electrospinning/electrospraying. J Mater Sci: Mater Med 27:143
  15. Jennes et al. (2012) Fabrication of three-dimensional electrospun microscope using phase modulated femtosecond laser pulses https://doi.org/10.1016/j.matlet.2011.09.015
  16. John and Chamberlain (1978) Mechanical properties of coral skeleton: compressive strength and its adaptive significance 4(4) (pp. 419-435) https://doi.org/10.1017/S0094837300006163
  17. Karageorgiou and Kaplan (2005) Porosity of 3D biomaterial scaffolds and osteogenesis (pp. 5474-5491) https://doi.org/10.1016/j.biomaterials.2005.02.002
  18. Kazemnejad S (2009) Hepatic tissue engineering using scaffold, state of the art. Avicenna J Med Biotechnol 1(3):135–145
  19. Khorshidi et al. (2015) A review of key challenges of electrospun scaffolds for tissue-engineering applications https://doi.org/10.1002/term.1978
  20. Kidoaki et al. (2005) Mesoscopic spatial designs of nano- and microfiber meshes for tissue-engineering matrix and scaffold based on newly devised multilayering and mixing electrospinning techniques (pp. 37-46) https://doi.org/10.1016/j.biomaterials.2004.01.063
  21. Kim and Lee (2011) Novel approach to the fabrication of an artificial small bone using a combination of sponge replica and electrospinning methods https://doi.org/10.1088/1468-6996/12/3/035002
  22. Kim et al. (2014) Fabrication of three-dimensional poly(lactic-co-glycolic acid) mesh by electrospinning using different solvents with dry ice https://doi.org/10.1007/s13233-014-2060-7
  23. Koh et al. (2010) Ramakrishna S. In vivo study on novel nanofibrous intra-luminal guidance channels to promote nerve regeneration https://doi.org/10.1088/1741-2560/7/4/046003
  24. Lee et al. (2011) highly porous electrospun nanofibers enhanced by ultrasonication for improved cellular infiltration https://doi.org/10.1089/ten.tea.2010.0709
  25. Leung et al. (2012) Fabrication of 3D electrospun structures from poly(lactide-co-glycolide acid)-nano-hydroxyapatite composites https://doi.org/10.1002/polb.22396
  26. Li et al. (2003) Biological response of chondrocytes cultured in three-dimensional nanofibrous poly(epsilon-caprolactone) scaffolds (pp. 1105-1114) https://doi.org/10.1002/jbm.a.10101
  27. Loh and Choong (2013) Three-dimensional scaffolds for tissue engineering applications: role of porosity and pore size 19(6) (pp. 485-502) https://doi.org/10.1089/ten.teb.2012.0437
  28. Madurantakam et al. (2013) Compression of multilayered composite electrospun scaffolds: a novel strategy to rapidly enhance mechanical properties and three dimensionality of bone scaffolds
  29. Martini FH, Timmons MJ, Tallitsch RB (2011) Human anatomy, 7th ed. Pearson, London. ISBN:10:0321688155ISBN 13:9780321688156
  30. Milleret et al. (2011) Tuning electrospinning parameters for production of 3D-fiber fleeces with increased porosity for soft tissue engineering applications https://doi.org/10.22203/eCM.v021a22
  31. Nam et al. (2007) Improved cellular infiltration in electrospun fiber via engineered porosity (pp. 2249-2257) https://doi.org/10.1089/ten.2006.0306
  32. Netter FH (1987) Musculoskeletal system: anatomy, physiology, and metabolic disorders. Ciba-Geigy Corporation, Summit, New Jersey
  33. Nguyen et al. (2012) Enhanced osteogenic differentiation with 3D electrospun nanofibrous scaffolds 7(10) (pp. 1561-1575) https://doi.org/10.2217/nnm.12.41
  34. Pezeshki-Modaress et al. (2014) Cell loaded gelatin/chitosan scaffolds fabricated by salt-leaching/lyophilization for skin tissue engineering: in vitro and in vivo study 102(11) (pp. 3908-3917) https://doi.org/10.1002/jbm.a.35054
  35. Pham et al. (2006) Electrospun poly(epsilon-caprolactone) microfiber and multilayer nanofiber/microfiber scaffolds: characterization of scaffolds and measurement of cellular infiltration (pp. 2796-2805) https://doi.org/10.1021/bm060680j
  36. Ru et al. (2013) A multifunctional electrospinning system for manufacturing diversified nanofibrous structures https://doi.org/10.1063/1.4819123
  37. Sawawi et al. (2013) Scission of electrospun polymer fibres by ultrasonication https://doi.org/10.1016/j.polymer.2013.05.060
  38. Shim et al. (2010) Novel three-dimensional scaffolds of poly(L-lactic acid) microfibers using electrospinning and mechanical expansion: fabrication and bone regeneration (pp. 150-160) https://doi.org/10.1002/jbm.b.31695
  39. Sun et al. (2012) Self-assembly of a three-dimensional fibrous polymer sponge by electrospinning https://doi.org/10.1039/c2nr11782g
  40. Szentivanyi et al. (2011) Electrospun cellular microenvironments: understanding controlled release and scaffold structure (pp. 209-220) https://doi.org/10.1016/j.addr.2010.12.002
  41. Thorvaldsson et al. (2008) Electrospinning of highly porous scaffolds for cartilage regeneration (pp. 1044-1049) https://doi.org/10.1021/bm701225a
  42. Tong and Wang (2013) A novel technique for the fabrication of 3D nanofibrous scaffolds using simultaneous positive voltage electrospinning and negative voltage electrospinning https://doi.org/10.1016/j.matlet.2012.12.015
  43. Wulkersdorfer et al. (2010) Bimodal porous scaffolds by sequential electrospinning of poly(glycolic acid) with sucrose particles https://doi.org/10.1155/2010/436178
  44. Xiao et al. (2010) Repair of orbital wall defects using biocoral scaffolds combinedwith bone marrow stem cells enhanced by human bonemorphogenetic protein-2 in a canine model (pp. 517-525)